[Shoutai Wan通过调节BRD4来激活Wnt4/β-catenin信号通路来改善子宫内膜分泌]
Shu-Chan Hu1, Zhao-Xi Zhang2, Yu Zhang1
1Institute of Integrative Medicine, Hebei University of Chinese Medicine Shijiazhuang 050200, China Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns Shijiazhuang 050200, China Hebei Collaborative Innovation Center of Integrated Traditional and Western Medicine on Reproductive Disease Shijiazhuang 050200, China.
概括
舒泰万 (STW) 通过激活Wnt4/β-catenin通路通过含原蛋白4 (BRD4) 激活,在复发性自发性流产 (RSA) 模型中增强子宫内膜分泌. 这促进了成功怀孕的关键蛋白质表达.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 复发性自发性流产 (RSA) 在生殖医学中构成了重大挑战.
- 子宫内膜分泌是成功植入和维持怀孕的关键过程.
- Wnt4/β-catenin信号通路和含原蛋白蛋白4 (BRD4) 都与决定化有关.
研究的目的:
- 调查Shoutai Wan (STW) 对BRD4和Wnt4/β-catenin通路的影响.
- 探索STW在促进RSA治疗中的子宫内膜决定化的机制.
- 在RSA小鼠模型中评估STW的治疗潜力.
主要方法:
- 建立了RSA的小鼠模型,并用STW或dydrogesterone进行治疗.
- 使用人类子宫内膜层细胞 (HESC) 的体外模型和操纵的BRD4表达.
- 通过HE染色来评估子宫内膜分泌;使用西斑和免疫组织化学测量蛋白质水平 (BRD4,IGFBP-1,PRL,Wnt4,β-catenin).
主要成果:
- 在RSA小鼠中,STW显著改善了子宫内膜分泌.
- STW治疗增加了益乳素 (PRL) 和胰岛素样生长因子结合蛋白-1 (IGFBP-1) 的表达.
- 在体外和体外模型中,STW激活了BRD4介导的Wnt4/β-catenin信号通路.
结论:
- 在重复性自发性流产的背景下,Shoutai Wan有效地促进子宫内膜分泌.
- STW的机制涉及通过BRD4.4激活Wnt4/β-catenin信号通路.
- STW显示出作为治疗治疗复发性自发性流产的治疗剂的潜力.
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