[通过CcWRKY40转录因子调节类生物合成 在Coptis chinensis中]
Mu He1, Jing-Jing Yu1, Ke-di Zhang1
1Engineering Research Center of Coptis Development and Utilization (Ministry of Education), College of Pharmaceutical Sciences, Southwest University Chongqing 400715, China.
概括
这项研究确定CcWRKY40是调节Coptis chinensis中类生物合成的关键转录因子. CcWRKY40激活了CcCNMT和Cc4'OMT基因的转录,这对化物产生至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物化学 生物化学
- 遗传学 遗传学 是一个
背景情况:
- 在Coptis chinensis中类生物合成的理解是部分的,但调节机制需要进一步的研究.
- 甲基斯酸盐 (MeJA) 诱导了参与Coptis chinensis二次代谢的基因的表达.
- 转录因子在调节植物的二次代谢途径中起着至关重要的作用.
研究的目的:
- 鉴定和表征一种转录因子,该因子参与了C. chinensis.中类生物合成调节.
- 阐明CcWRKY40在化物生物合成途径中的关键基因上的调节机制.
- 为了解CcWRKY40在C. chinensis化物生产中的作用提供基础.
主要方法:
- 基因克隆,生物信息学分析和亚细胞局部化预测 (烟草转化).
- 基因表达分析 (RT-PCR) 和酵母单杂交试验用于促进体相互作用.
- 双露西法酶记者测定转录激活和分子对接,用于结合部位的预测.
主要成果:
- 克隆了CcWRKY40基因 (951 bp ORF, 316 个氨基酸) 并将其定位到核中.
- CcWRKY40在C. chinensis.的细根中表达最高.
- CcWRKY40直接与CcCNMT和Cc4'OMT的促进体结合并激活,结合位预测在RKYGQK域中.
结论:
- CcWRKY40作为CcCNMT和Cc4'OMT的转录激活剂,这是C. chinensis类生物合成中的关键基因.
- 这项研究确立了CcWRKY40作为化物生物合成途径的重要调节者.
- 这些发现有助于更深入地了解C. chinensis二次代谢中的转录调节.
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