植物免疫激活内菌诱导培养植物细胞的动态代谢变化,而不会抑制它们的生长
Yui Aikawa1, Ayano Yabuuchi1, Hiroki Kaneko1
1Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba, Japan.
Microbial biotechnology
|January 9, 2026
概括
激活植物免疫力的内菌增强植物细胞代谢,而不会阻碍生长. 这些细菌诱导防御基因表达和化合物生产,为植物化学合成提供了潜力.
科学领域:
- 植物生物技术 植物生物技术
- 微生物生物技术 微生物生物技术
- 代谢学 代谢学 代谢学
背景情况:
- 与植物细胞共同培养的微生物可以诱导生物活性化合物合成.
- 微生物共同培养通常会抑制植物细胞的生长,限制它们的应用.
- 激活植物免疫力的内性细菌显示出克服生长抑制的前景.
研究的目的:
- 研究植物免疫激活内菌在培养植物细胞中诱导代谢变化的潜力.
- 为了评估Delftia sp.的影响. 关于烟草BY-2细胞生长和新陈代谢的BR1R-2.
- 为了确定细菌诱导的代谢变化是否需要身体接触.
主要方法:
- 烟草BY-2细胞与Delftia sp.的共同培养 它们包括BR1R-2和大肠杆菌.
- 分析细胞生长和代谢概况.
- 对与防御相关的基因进行基因表达分析.
- 共同培养实验使用过系统来控制身体接触.
- 烟草BY-2和阿拉比多普西斯T87细胞与Pseudomonas sp.的共同培养 这就是RS1P-1.
主要成果:
- 德尔菲亚种 (Delftia sp.) 是一种植物. 与大肠杆菌不同的是,BR1R-2并没有抑制烟草BY-2细胞的生长.
- 与BR1R-2共同培养显著改变了BY-2细胞的代谢概况.
- BR1R-2诱导了与防御相关的基因表达和抗菌化合物生产.
- 代谢变化需要BY-2细胞和BR1R-2细胞之间的物理接触.
- 伪omonas sp. 这种病毒是假的. RS1P-1还在没有增长抑制的情况下诱导烟草BY-2和阿拉比多普西斯T87细胞的代谢变化.
结论:
- 植物免疫激活的内菌可以用于修改植物细胞代谢.
- 这些细菌提供了一种可行的策略,用于增强植物细胞培养中的有价值植物化学物质的生产.
- 内生菌体细菌是植物代谢工程的一个有前途的工具.
关键词:
阿拉比多普西斯 (Arabidopsis) 是一种植物.塞尔维亚的海湾地区.伪omonas 是一个类型的.乙烯基烯 (acetophenone) 是一种共同文化是一种共同文化.诱导的诱导方式它们的内基质 (endophyte) 是:烟草烟草的烟草烟草是什么更多相关视频
相关概念视频
Defenses Against Pathogens and Herbivores
29.5K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
29.5K
Plant Tissue Culture
40.1K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
40.1K
Transgenic Plants
8.4K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.4K
Cell Signaling in Plants
6.1K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.1K
Cell Culture
21.9K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
21.9K


