来自Helicobacter pylori的O-抗原八分糖的化学酶合成和抗原性评估

相关实验视频

Updated: Jan 13, 2026

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

Wei Liu¹, Wei Zhang^1,2, Zhuojia Xu¹

¹State Key Laboratory of Chemical Biology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.

Angewandte Chemie (International ed. in English)

|January 9, 2026

概括

研究人员开发了Helicobacter pylori O-抗原的新型化学酶合成方法,确定了开发基于碳水化合物的H. pylori感染疫苗的关键无糖糖类表位.

科学领域:

碳水化合物化学碳水化合物化学
疫苗开发疫苗开发
微生物学微生物学

背景情况:

杆菌感染是一个全球性的健康问题,与胃癌和抗生素耐药性有关.
以碳水化合物为基础的针对脂多糖 (LPS) O抗原的疫苗为抗生素提供了一个有希望的替代品.
开发有效的疫苗需要了解H.pylori O-抗原的免疫性.

研究的目的:

通过综合化酶方法合成H. pylori SS1 LPS O-抗原及其碎片.
评估合成的O-抗原及其子单位的抗原性.
为了确定潜在的疫苗开发的关键表征.

主要方法:

具有独特结构图案的十糖前体的模块化化学合成.
酶延长用于精确安装杂交的易斯抗原部分和fucosidic链接.
具有挑战性的1,2-cis-glycosidic链接的立体选择性构造.
使用H. pylori感染患者血清进行糖甘微阵列查.

主要成果:

成功合成了八度十糖糖酸O抗原和相关碎片.
识别一个无糖糖体作为一个更简单的,关键的表位.
对复杂的糖甘合成的化学酶策略的演示.
在特定地点安装五个具有挑战性的1,2-cis-glycosidic链接.

结论:

合成的未脱糖糖化物是H. pylori疫苗开发的有希望的目标.
综合的化学酶策略使复杂的细菌甘氨酸的合成成为可能.
这项工作为开发针对H. pylori的新型碳水化合物基础疫苗提供了基础.

关键词:

抗原性评价的抗原性评估.细菌的寡糖类碳水化合物碳水化合物化学酶合成的合成方法葡萄糖基酶化是什么? 葡萄糖基酶化

更多相关视频

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Published on: December 19, 2020

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

相关概念视频

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...

Antigens Involved in Adaptive Immunity

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...