综合性元基因组,代谢组和转录组分析揭示了可可发酵中定义的起始尾酒所驱动的风味发展
Haode Chang1, Quanmiao Zhang2, Pégnonsienrè Lacina Soro3
1Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, 571533, Hainan, China; Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops, 571533, Hainan, China; Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, 150030 Harbin, China.
Food research international (Ottawa, Ont.)
|January 9, 2026
概括
定义的四种微生物的起始培养显著改善了Trinitario可可发酵. 这种方法提高了香味特征和发酵的一致性,为食品生产中的受控微生物联盟铺平了道路.
科学领域:
- 食品微生物学 食品微生物学
- 发酵科学 发酵科学
- 代谢学与基因组学
背景情况:
- 可可发酵对于香味的发展至关重要,但往往缺乏一致性.
- 定义的起始培养为控制微生物动态和增强发酵结果提供了潜在的解决方案.
研究的目的:
- 调查定义的四个菌株初始培养对Trinitario可可发酵的影响.
- 通过使用多组的方法来阐明发酵过程中的微生物继承,代谢物概况和基因表达.
主要方法:
- 多原子分析整合了射门元基因组学,代谢组学 (LC-MS,GC-MS) 和转录组学.
- 在注射可可发酵中监测微生物群落动态和代谢物生产.
主要成果:
- 启动注射加速了发酵,稳定了微生物的继承,引入的菌株占主导地位.
- 观察到关键的气味挥发物 (乙醇,3-甲基乙酸,3-甲基乙酸,delta-decalactone) 的产生增加.
- 转基因组学和转录组学揭示了增强的碳水化合物利用,氨基酸代谢和乙酸生物合成,其中Saccharomyces cerevisiae和Hanseniaspora uvarum具有特定的作用.
结论:
- 定义的初始培养可以显著提高可可发酵的一致性和香味质量.
- 这项研究为设计可控发酵过程的功能性微生物联盟提供了基础.
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