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转录的线粒体逆行控制与呼吸应激,代谢适应和发芽酵母中的毒性演变
Karolina Łabędzka-Dmoch1, Thi Hoang Diu Bui1, Jakub Piątkowski1
1University of Warsaw, Faculty of Biology, Institute of Genetics and Biotechnology, Warsaw, Poland.
在Candida albicans中Rtg通路适应新陈代谢以应对呼吸应激,与Saccharomyces cerevisiae相比具有独特的目标基因. 这个古老的系统系统.
科学领域:
- * 分子和进化生物学
- * 酵母遗传学公司
- * 细胞代谢 细胞代谢
背景情况:
- *Rtg路径 (Rtg1/Rtg3转录因子) 调节细胞代谢,以应对Saccharomyces cerevisiae中的线粒体呼吸状态.
- *研究Candida albicans中的这种途径,一种基因学上遥远的酵母,为其进化适应提供了洞察力.
- * 了解Rtg路径演变揭示了酵母物种之间保存和分离的机制.
研究的目的:
- * 研究Candida albicans中Rtg通路的功能和演变.
- * 鉴定和描述由C. albicans中Rtg通路调节的目标基因.
- * 追踪Rtg通路组件和Saccharomycotina亚族中的基因的进化史.
主要方法:
- *对Rtg通路组件和基因进行比较基因组学和遗传学分析.
- *使用Candida albicans的呼吸缺陷突变体进行的功能研究.
- *对在芽的酵母菌系中发生的基因复制和损失事件的分析.
主要成果:
- *C. albicans中的Rtg通路调节了适应呼吸功能障碍的调节基因,调节了参与替代呼吸,氧化应激和线粒的基因.
- *C. albicans中Rtg标基因的谱系与S. cerevisiae有显著的差异.
- *Rtg路径起源于Saccharomycotina通过基因重复,其中Rtg2和Mks1扮演的角色可能存在于共同祖先中.
- * Rtg2和Mks1在大多数Serinales中丢失,替代氧化酶 (Aox) 基因经历了独立的重复或损失,与复杂I存在相关.
结论:
- *Rtg通路是酵母中代谢应激反应的保存但可适应的系统.
- * 进化分析揭示了在Rtg通路调节和基因谱中发生的谱系特异性适应.
- * 替代氧化酶和线粒体复合体I的共同进化突出了在酵母进化的氧化应激管理策略.
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