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一种简单,敏感和快速的生物分析方法,用于在人体血中量化大麻醇 (CBD),7-OH-CBD和7-COOH-CBD

Nattapon Jaisupa¹, Michael Ashton¹, Sofia Birgersson¹

¹Unit for Pharmacokinetics and Drug Metabolism, Department of Pharmacology, Institute of Neuroscience and Physiology, University of Gothenburg, Gothenburg, Sweden.

|January 9, 2026

概括

一种新的,简单的方法准确地测量了人体血中的大麻素 (CBD) 和其代谢物. 这种可靠的技术非常适合药理动力学研究和常规临床使用.

科学领域:

药理学药理学是一个学科.
分析化学分析化学
生物化学生物化学

背景情况:

精确测量血大麻素 (CBD) 和其代谢物对于药理动力学研究至关重要.
量化CBD及其在血中的代谢物的现有方法可能是复杂的.
需要一种简单,强大的生物分析方法来同时量化.

研究的目的:

开发一种简单,快速和强大的生物分析方法,用于在人体血中量化CBD,7-OH-CBD和7-COOH-CBD.
为了优化样本准备和分析参数,以准确量化.
为了验证方法的各种参数,包括稳定性和准确性.

主要方法:

评估了用于样本制备的各种溶剂系统,选择基于乙二的蛋白质沉.
优化了分析参数,并对灵敏度,准确度,精度,选择性,特异性,转载率,稀释完整性和稳定性进行了验证.
利用负电离模式和一个C18柱与同位素移动相在3.5分钟内进行分离.

主要成果:

使用乙尼的简单蛋白质沉方法被证明是样本准备的最佳方法.
该方法在3.5分钟内实现了负电离模式下分析物的完全分离.
开发的方法符合所有验证标准,在各种储存条件下显示稳定性,在冷藏两个月后,7-COOH-CBD的含量略有下降.

结论:

介绍了一种简单,快速和强大的生物分析方法,用于同时定量CBD及其主要代谢物.
该方法因其可靠性和效率而适用于常规临床使用和药理动力学应用.
能够在临床相关的血度下准确测量CBD和代谢物.

关键词:

大麻二醇 (Cannabidiol) 是一种在HPLC-MS/MS中使用.生物分析方法生物分析方法血中的度是血中的度.验证验证的时间

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