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相关概念视频

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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In-vitro Mutagenesis01:16

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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相关实验视频

Updated: Jan 13, 2026

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

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在vivo中剖析Hip1功能,使用条件淘汰鼠标模型.

Ryuma Haraguchi1, Riko Kitazawa1,2, Yuta Yanagihara3

  • 1Department of Molecular Pathology, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime 791-0295, Japan.

Acta histochemica et cytochemica
|January 9, 2026
PubMed
概括
此摘要是机器生成的。

一个新的条件淘汰赛小鼠模型,用于鼠相互作用蛋白1 (Hhip1) 揭示了它在维护产后生长板架构和防止长骨过度生长方面的关键作用.

关键词:
H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1H1有条件的淘汰赛.刺信号传输 刺信号传输长骨的发育 长骨的发育肺部形态发生的肺部形态发生.

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Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
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Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue

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Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
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Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

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相关实验视频

Last Updated: Jan 13, 2026

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

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Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
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Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue

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Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
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Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

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科学领域:

  • 发展生物学 发展生物学
  • 遗传学 是一个遗传学.
  • 分子信号传输的方法

背景情况:

  • 刺 (Hh) 信号通路对于发育和平衡至关重要.
  • 刺相互作用蛋白1 (Hhip1) 是Hh信号的负调节者.
  • 之前的研究受到全球Hhip1淘汰赛模型中围产死亡率的限制.

研究的目的:

  • 为研究产后Hhip1功能的条件淘汰模型开发.
  • 研究Hhip1在产后发育,特别是肢体发育中的作用.
  • 为了探索Hh信号的时空调节.

主要方法:

  • 使用CRISPR/Cas9.9,生成一个有条件的Hhip1等位基因 (Hhip1^flox).
  • 创建中介切除以创建一个有条件的零等位基因 (Hhip1^ex2Δ).
  • 使用CMV-Cre进行生殖线重组和Prx1-Cre进行特定肢体的淘汰.

主要成果:

  • Hhip1^flox模型成功地重复了已知的Hhip1淘汰现象型.
  • 特定于四肢的Hhip1条件淘汰赛小鼠表现出渐进的生长板扩张和长骨过度生长.
  • 在条件淘汰赛小鼠中观察到Gli1表达的持续上调.

结论:

  • Hhip1^flox模型是Hhip1的组织和阶段特定功能研究的宝贵工具.
  • 1在维持产后生长板架构方面发挥着以前未知的作用.
  • 该模型提供了对发育和疾病中的Hh信号调节的见解.