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在小鼠中,COPS5对塞尔托利细胞功能和男性生育能力至关重要

Changmin Niu, Tao Li, Wei Li

bioRxiv : the preprint server for biology

|January 9, 2026

概括

COP9信号酶子单元5 (COPS5) 对于男性生育能力至关重要. 塞尔托利细胞中COPS5的丧失会破坏丸功能,通过损害细胞极性和血液丸屏障,导致不孕.

科学领域:

生殖生物学生殖生物学
细胞生物学细胞生物学
分子内分泌学分子内分泌学

背景情况:

COP9信号酶子单元5 (COPS5) 调节依赖于乌比奎的过程,在生殖细胞中至关重要.
对于精子生成至关重要的塞尔托利细胞中COPS5的功能以前是未知的.
全球警察5淘汰赛是致命的胚胎,突出其关键的角色.

研究的目的:

为了研究COPS5在男性生殖中在塞尔托利细胞中的重要作用.
确定塞尔托利细胞特异性COPS5切除对男性生育能力和丸功能的影响.

主要方法:

产生特定于塞尔托利细胞的Cops5淘汰赛小鼠模型.
在突变小鼠中评估男性生育能力,丸组织学和体重.
分析塞尔托利细胞极性,血液测试屏障 (BTB) 完整性和细胞间通信.

主要成果:

塞尔托利细胞特异性Cops5淘汰导致了依赖年龄的男性不孕症.
突变小鼠表现出丸缩,精子数量减少和管道失调.
丢失COPS5破坏了塞尔托利细胞极性,损害了BTB完整性 (ZO-1),并损害了间隙结通信 (Connexin 43).

结论:

COPS5对于塞尔托利细胞功能和男性生育能力是不可或缺的.
失去COPS5会破坏塞尔托利细胞极性和BTB完整性,导致精子生成失败.
COPS5是丸体内的一个新型,必不可少的调节器,用于男性生殖.

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