相关实验视频

Updated: Jan 13, 2026

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis

Published on: October 21, 2015

在丸发育in vitro的过程中,不同的视网酸反应in vitro

Brad C Hansen, Sarah A Hatem, Lucas A Huang

bioRxiv : the preprint server for biology

|January 9, 2026

概括

纳米分子异氨酸驱动精子分化标志物Stra8在体外表达. 来自老鼠丸的复杂体外模型 (CIVM) 揭示了介质补充剂对精子干细胞表型的影响.

科学领域:

生殖生物学和干细胞研究.
研究精子生成的细胞动态.

背景情况:

精子的分化依赖于精确的酶和信号分子活动.
试验室模型提供了关于Sertoli和精子干细胞相互作用的见解,这对于治疗不孕症至关重要,就像非阻断性亚精子症一样.

研究的目的:

为了在体外研究异氨酸对精子分化标记物的作用.
为了比较来自老鼠丸不同发育阶段的复杂体外模型 (CIVMs).
评估介质补充对精子干细胞表型的影响.

主要方法:

使用复杂的体外模型 (CIVM),用从出生后第5天和第10天的老鼠丸中获得的细胞进行播种.
给出不同剂量的异丁氨酸,以评估其对Stra8表达的影响.
分析了Stra8和Plzf的表达,以应对媒体补充.

主要成果:

纳米分子异氨酸诱导的剂量依赖的Stra8表达在体外.
CIVM 证明了将视网醇代谢为生物活性视网类药物的能力.
媒体补充剂显著改变了Stra8和Plzf的表达,表明对分化和不分化状态的影响.

结论:

在纳米分子度下,异氨酸可以有效地推动早期的精子分化在体外.
复杂的体外模型是研究精子和视网膜体代谢的宝贵工具.
标准介质组件可以影响精子干细胞的行为,需要在实验设计中仔细考虑.

更多相关视频

Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay

Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay

Published on: September 6, 2016

Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids

Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids

Published on: October 7, 2020

相关概念视频

Testes: Histology

Testes: Histology

A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
The spermatogenic cells, responsible for producing sperm, are...

Development of the Sexual Organs in the Embryo and Fetus

Development of the Sexual Organs in the Embryo and Fetus

Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...