相关实验视频

Updated: Jan 13, 2026

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
12:21

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Published on: December 13, 2014

13.0K

基因组停止密码子扫描揭示了无意义介导的mRNA衰变的定量原理

Michael A Cortázar, Jacob Schmidt, Iman Egab

    bioRxiv : the preprint server for biology
    |January 9, 2026
    PubMed
    概括

    无意中介的mRNA衰变 (NMD) 通过降解错误的转录来对疾病产生定量影响. 这项研究揭示了超越当前模型的复杂NMD规则,为变体解释提供了新的见解.

    更多相关视频

    Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts07:03

    Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

    Published on: January 2, 2018

    6.6K
    Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems06:18

    Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems

    Published on: April 26, 2019

    6.4K

    相关概念视频

    Nonsense-mediated mRNA Decay02:27

    Nonsense-mediated mRNA Decay

    The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
    Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
    11.7K
    Nonsense-mediated mRNA Decay02:27

    Nonsense-mediated mRNA Decay

    3.3K
    Nuclear Export of mRNA02:31

    Nuclear Export of mRNA

    Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
    8.7K
    Nuclear Export of mRNA02:31

    Nuclear Export of mRNA

    5.2K
    mRNA Stability and Gene Expression02:51

    mRNA Stability and Gene Expression

    The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
    Cis-acting Elements involved in mRNA stability
    6.5K
    mRNA Stability and Gene Expression02:51

    mRNA Stability and Gene Expression

    3.4K