直接RNA测序揭示了在50S生物发生过程中依赖于压力和特定途径的rRNA修饰重编程
bioRxiv : the preprint server for biology
|January 9, 2026
概括
核糖体组装涉及重要的RNA修饰. 这项研究使用直接RNA测序来分析大肠杆菌的修饰,揭示了特定组装途径中变化的伪乌里丁合并,表明了核糖体生物发生的新调节层.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生化学
背景情况:
- 核糖体生物发生是一个复杂的,多步骤的过程,涉及核糖体RNA (rRNA) 的修改和处理.
- 核糖体RNA的修饰对于核糖体组装和功能至关重要,但它们在组装过程中的动态调节尚未完全理解.
研究的目的:
- 为了研究DbpA酶活性对23SrRNA修饰和3'-end处理在核糖体组装过程中的影响.
- 在成熟的50S大子单元 (LSU) 和独特的LSU组装中间体中分析rRNA修饰.
主要方法:
- 利用牛津纳米孔直接RNA测序来同时检测和量化23SrRNA修饰.
- 在野生型和突变型 (R331A DbpA) Escherichia coli 细胞中分析了 23S 和 5S rRNA 的 3'-end 处理.
- 在成熟的50S LSU和35S/45S组装中间体中比较rRNA修饰模式.
主要成果:
- 大多数23SrRNA修饰都被类似地纳入中间体和成熟子单元,不论DbpA活性如何.
- N2-甲基氨酸2507和伪氨酸 (Ψ) 2508显示,从R331A DbpA表达细胞中改变了50SLSU的整合.
- 与中间体和野生型50S子单元相比,从R331A DbpA细胞的50S子单元中减少了Psy2608的纳入,这表明路径特定的调节.
结论:
- 在核糖体组装过程中,DbpA酶活性会影响特定的23SrRNA修饰.
- 伪乌里丁2608的整合被选择性地通过不同的 in vivo 核糖体组装路径重新编程.
- 这些发现揭示了由rRNA修饰动态控制的核糖体生物发生的额外调节层.
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