相关实验视频

Updated: Jan 13, 2026

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
07:26

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Published on: December 26, 2020

4.4K

在保存的氨酸沙漠中,氨酸缺乏对EEL-1/HUWE1来说至关重要,以支持无处不在蛋白质酶体系统功能

Katherine S Yanagi, Brenda J Chen, Sheikh Omar Kunjo

    bioRxiv : the preprint server for biology
    |January 9, 2026
    PubMed
    概括

    EEL-1蛋白通过增强基质的无化来维持细胞蛋白平衡,这对无蛋白酶系统 (UPS) 至关重要. 这一发现为与UPS功能障碍相关的疾病提供了新的治疗点.

    更多相关视频

    In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones11:36

    In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

    Published on: July 25, 2019

    11.4K
    A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli11:08

    A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

    Published on: December 9, 2017

    7.4K

    相关概念视频

    Lysosomal Hydrolases01:22

    Lysosomal Hydrolases

    Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
    4.4K
    Regulated Protein Degradation02:58

    Regulated Protein Degradation

    It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
    Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
    8.7K
    The Proteasome01:13

    The Proteasome

    Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
    In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
    1.6K
    The Proteasome02:18

    The Proteasome

    Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
    In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
    10.0K
    Covalently Linked Protein Regulators02:04

    Covalently Linked Protein Regulators

    Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
    These groups modify specific amino acids in a protein....
    8.6K
    Lysosomes01:31

    Lysosomes

    Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
    25.3K