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深紫外线图形口袋镜 (DART):中等尺度无透镜分子成像,使用无标签的光谱对比度

Ruihai Wang¹, Qianhao Zhao¹, Julia Quinn²

¹Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269 USA.

eLight

|January 9, 2026

概括

一种新的手持式深紫外线图形口袋镜 (DART) 通过使用内在光谱对比,使无标签的分子成像成为可能. 这项技术可以在没有外部标签的广大区域内提供高分辨率的核酸和蛋白质映射.

科学领域:

生物光子学生物光子学
显微镜的使用方法
频谱学是一种光谱学.

背景情况:

传统的中尺度生物成像在分辨率,视野,视野深度和分子特异性方面面临着权衡,通常需要外部标签.
开发无标签技术对于推进生物样本表征至关重要.

研究的目的:

推出深紫外线图形口袋镜 (DART),这是一个用于无标签分子成像的手持平台.
为了证明DART的高分辨率能力,使用内在光谱对比度进行大尺度成像.

主要方法:

使用深紫外线 (DUV) 光来利用生物分子的自然吸收指纹.
结合了无镜头图形显微镜与虚拟错误箱方法来消除文物.
采用差分光谱成像在DUV范围用于定量分子映射.

主要成果:

在厘米尺度的区域内达到低至308nm线宽的分辨率,具有毫米尺度的深度.
定量地绘制核酸和蛋白质分布的图谱,具有femtogram的灵敏度.
在各种生物标本中展示无标签的分子对比,包括组织部分,细胞病理样本,血液细胞和神经群体.

结论:

通过利用内在光谱对比度,DART提供了高准确度,无标签的分子成像.
便携式平台集成了高分辨率的分子映射与广泛的中等尺度成像.
在不同的环境中为快速的临床诊断,组织分析和生物表征开辟了新的途径.

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