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使用REFLEX进行大规模可扩展的T细胞谱的单细胞多原子分析

Peter Skene, Matthew Hart, Zach Thomson

Research square

|January 9, 2026

概括

我们开发了REFLEX,这是一种用于单细胞免疫分析的新型高通量方法. 这种技术使得可扩展的,具有成本效益的多原子分析与配对的T细胞受体测序,推进适应性免疫研究.

科学领域:

免疫学免疫学免疫学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

了解T细胞异质性对于分析免疫反应至关重要.
目前的单细胞技术缺乏复杂的免疫谱系研究的吞吐量.

研究的目的:

推出REFLEX,一种新,可扩展和成本效益高的单细胞方法.
为了实现高通量多原子分析与配对的T细胞受体 (TCR) 测序.

主要方法:

REFLEX集成了现场反转录和样本复杂化条形码.
它与10x FLEX平台无结合,用于增强数据捕获.
选涉及CMV脉冲T细胞扩张的200多万个细胞.

主要成果:

成功捕获了140万个T细胞的TCR序列和多原子数据.
确定了许多可谓的新型CMV反应性克隆类型.
在T细胞谱系分析中证明了前所未有的规模和深度.

结论:

REFLEX显著提高了单细胞免疫分析的规模和深度.
该方法为T细胞介导的适应性免疫提供了变革性的见解.
REFLEX有助于发现抗原特异性T细胞反应.

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