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对法医学DNA样本的ForenSeqTM MainstAY工作流程的评估

Lucio L Avellaneda¹, Ryan M Gutierrez¹, Damani T Johnson¹

¹Department of Forensic Science, College of Criminal Justice, Sam Houston State University, Huntsville, Texas, USA.

Journal of forensic sciences

|January 9, 2026

概括

在法医DNA分析中,ForenSeqTM MainstAY套件显示出高可靠性,在具有挑战性的样品中,其表现优于毛细管电泳. 工作流调整可以优化高通量测序环境中的性能.

科学领域:

法医科学法医科学法医科学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

在MiSeq FGx®测序系统上的ForenSeqTM MainstAY套件被批准用于国家DNA索引系统 (NDIS) 的使用.
对法医实验室来说,评估其性能至关重要.

研究的目的:

为了全面评估ForenSeqTM MainstAY化学的性能和局限性.
为法医实验室采用本套件提供实际指导.

主要方法:

进行了基线性能,灵敏度和可重复性研究.
分析了具有挑战性的案例样本,比较了不同的图书馆准备方法,PCR缓冲器和流细胞类型.
与毛细管电泳 (CE) 进行了比较.

主要成果:

该套件表现出高可靠性和在用户之间一致的性能,最小的位点下降到62 pg.
它在低输入和降解样本中表现优于CE.
在较高的度下观察到限制,增加的样本数量减少了退化样本的读数.

结论:

该ForenSeqTMMainstAY套件可靠用于法医DNA分析,特别是对于具有挑战性的样品.
工作流程的战略调整,如分批降解样本或降低性,可以减轻性能限制.
自动化图书馆准备显示了可比或改进的结果,没有交叉污染.

关键词:

在 ForenSeqTM 主要工具包中.MiSeq FGx® 测序系统的测序系统自动化自动化自动化自动化退化的DNA退化.这是下一代测序.复杂性复杂性的短串联重复重复的重复.

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