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Updated: Jan 13, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

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Published on: August 3, 2018

使用错误纠正的测序来评估molnupiravir在人类中的致变性

Page B McKinzie¹, Rutu R Valapil², Emily L Heil²

¹Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson AR, 72116.

|January 9, 2026

概括

摩尔努皮拉维尔 (MOV) 是一种口服的COVID-19治疗药物,它被评估是否具有突变效应. 这项研究发现,与对照组相比,用Molnupiravir治疗的患者的突变没有增加,这表明它没有突变后果.

科学领域:

病毒学病毒学
遗传学是一个遗传学.
药理学药理学是一个学科.

背景情况:

莫尔努皮拉维尔 (MOV) 是N-基丁 (NHC) 的口服前药物,用于治疗SARS-CoV-2感染.
虽然MOV和NHC在细胞培养中表现出变异性,但体内数据尚未确定.
开发了一种新的纠错测序 (ECS) 方法,用于检测人体组织样本中的突变.

研究的目的:

评估Molnupiravir在接受COVID-19治疗的患者中的体内突变性.
为了比较接受MOV的患者与匹配的对照组的突变频率和光谱.

主要方法:

招募了两组COVID-19患者:一个接受MOV治疗,一个没有 (匹配的对照).
利用经过验证的错误纠正测序 (ECS) 方法分析外围血液核细胞中的基对替代.
在MOV和对照组之间比较突变频率,类型和光谱.

主要成果:

在MOV和对照组之间没有观察到突变频率,类型或光谱的显著差异.
根据细胞培养数据,MOV组的突变光谱没有显示出MOV或NHC暴露所期望的特征.
该研究没有发现来自授权剂量Molnupiravir的突变效应的证据.

结论:

对于COVID-19治疗的授权剂量Molnupiravir似乎不会在患者中引起可检测的突变.
开发的ECS方法是评估各种实验环境中的体内突变发生的有价值工具.

关键词:

这就是HiFi测序.N-基胺 (NHC) 是一种基胺.突变的签名突变的签名突变频谱中的突变周围血液周围血液单个基对替换的替代方法

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