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来自牛奶的细胞外囊泡保护牛卵管上皮细胞免受氧化应激的影响

Seyed Omid Reza Mousavi¹, Qurat Ul Ain Reshi^1,2, Kasun Godakumara¹

¹Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.

|January 9, 2026

概括

牛奶衍生的细胞外囊泡 (mEVs) 保护牛卵管上皮细胞免受氧化应激. 软化不影响mEV的生物活性,表明它们的治疗潜力.

科学领域:

生殖生物学生殖生物学
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

细胞外囊泡 (EVs) 调解细胞间的通信,并显示出治疗的前景.
氧化应激 (OS) 对生殖健康产生负面影响.
牛卵管上皮细胞 (BOEC) 对于早期胚胎发育至关重要.

研究的目的:

研究乳制品EVs (mEVs) 对BOEC中化物诱导的OS的保护作用.
为了比较储存在-80°C的mEV与冷化的有效性.
探索mEV介导保护的潜在机制.

主要方法:

使用触流过方法分离mEV和藻类衍生的EV (aEV).
在OS条件下对BOEC进行不同度的EV处理.
分析细胞活力,OS标志物表达 (DDIT4,HIF1A) 和细胞迁移.
酶消化,miRNA和蛋白质载荷分析.

主要成果:

mEVs显著提高了BOEC的活力,除了在较高度的后处理.
储存方法 (冷与冷) 并没有影响mEV的疗效.
与aEVs相比,mEVs降低了OS标记物和改善了细胞迁移.
内部的EV载荷,特别是miRNA和蛋白质,可能会介导保护作用.

结论:

mEV有效地保护BOEC免受CoCl2诱导的OS.
淋化mEV保持其生物活性,促进储存和应用.
通过减轻氧化应激,mEVs代表了改善生殖健康的有希望的治疗策略.

关键词:

皮质细胞上皮质细胞上皮质细胞细胞外囊泡中的细胞外囊泡.这是一个小RNARNA.氧化应激是一种氧化应激.

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