核受体E75调节了Nilaparvata lugens中的变和卵巢发育
Xiaojuan Jiang1, Zhenghui Qiu1, Fuhong Wei1
1College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.
Insect molecular biology
|January 9, 2026
概括
尼拉帕尔瓦塔 (Nilaparvata lugens) E75 (NlE75) 对于棕色的发育至关重要,影响脂肪体和卵巢成熟. NlE75调节细胞循环和维原蛋白的表达,这对这种主要的水害虫的繁殖至关重要.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 生殖生物学 生殖生物学
背景情况:
- 棕色 (Nilaparvata lugens) 是一个重要的农业害虫.
- 核受体E75在各种生物体的发育和新陈代谢中起作用.
- 在昆虫发育,特别是繁殖方面,Nilaparvata lugens E75 (NlE75) 的特定功能尚不清楚.
研究的目的:
- 调查NlE75在尼拉帕尔瓦塔 Lugens的发展中的作用.
- 阐明NlE75在卵巢发育和繁殖中的功能背后的分子机制.
- 为了探索NlE75在调节维泰洛金和细胞循环基因中的参与.
主要方法:
- 通过RNA干扰 (RNAi) 来降低NlE75的表达.
- 观察女的化率和成年人的生殖参数.
- 脂肪体和卵巢形态的分析.
- 测量维特洛根因蛋白质水平.
- 目标基因的转录分析.
- 流细胞计测试以评估细胞循环的进展.
主要成果:
- 降低NlE75的调控降低了淋巴发育率,并显著损害了卵巢和丸的发育.
- 降低NlE75水平导致脂肪体形态变化,脂质含量降低,卵巢成熟延迟,影响卵子生产.
- NlE75 knockdown 降低了维泰洛金 (Vg),Vg-like1 和 Vg-like2 的表达,以及 Vg 蛋白质水平.
- NlE75调节细胞循环相关的基因,影响DNA复制和细胞循环进展,在卵巢细胞中观察到显著的干扰.
结论:
- 在Nilaparvata lugens中,NLE75对于正常发育,脂质代谢和生殖成功至关重要.
- NlE75在调节维特洛根因合成和卵巢细胞周期进展方面发挥着至关重要的作用.
- 这些发现揭示了E75在通过细胞循环调节协调昆虫发育和繁殖方面的新功能.
相关概念视频
Regulation of Nuclear Protein Sorting
3.2K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.2K
Transducer Mechanism: Nuclear Receptors
2.3K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
2.3K
Nuclear Export
4.8K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
4.8K
Receptor Downregulation in MVBs
2.8K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.8K
Background and Environment Affect Phenotype
7.4K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
7.4K


