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Updated: Jan 13, 2026

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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Published on: February 26, 2019

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对Cas9特异性的差异性Allosteric调制

Yuanhao Li1, Xin Li1, Yingjie Chen1

  • 1College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.

Journal of chemical theory and computation
|January 9, 2026
PubMed
概括

通过改变导向RNA (gRNA) 或Cas9.9来提高CRISPR-Cas9基因编辑精度. 这些变化虽然远离活性部位,但通过固定HNH核酶域来控制Cas9,从而增强编辑特异性.

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