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假定藻类碳水化合物硫转移酶的全基因组比较和遗传学分析

Chai-Ling Ho¹, Xuan-Zi Low², Wei-Kang Lee³

¹Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, 43400, Selangor, Malaysia. clho@upm.edu.my.

Journal of molecular evolution

|January 9, 2026

概括

在藻类中确定了碳水化合物硫转移酶 (CHST),揭示了不同的进化路径和基因转移事件. 这项研究提高了对不同藻类群体中硫酸多糖生产的理解.

科学领域:

海洋生物学海洋生物学
生物化学生物化学
进化生物学是进化的生物学.

背景情况:

碳水化合物硫转移酶 (CHSTs) 是合成藻类中硫酸多糖的关键酶.
CHST催化了硫基从PAPS转移到碳水化合物部分的过程,影响了SP的结构和功能.

研究的目的:

根据保护域 (PF00685, PF03567, PF06990, PF13469) 识别和描述假定藻类的CHST.
研究藻类CHSTs的进化史和植物遗传关系.
为了推断藻类CHSTs的基质特异性和硫化模式.

主要方法:

生物信息分析包括HMMER搜索和BLAST使用人类CHST作为查询.
遗传学分析以确定进化关系和潜在的水平基因转移 (HGT).
对藻类类 (Chlorophyta,Rhodophyta,Phaeophyceae) 的域结构和分布进行比较分析.

主要成果:

识别具有不同域组合的藻类CHST (PF00685/PF13469与PF03567/PF06990).
域分布与藻类类相关,这表明SP生物合成的谱系特异性进化.
植物遗传学分析支持藻类CHST进化中的多种类起源和显著的HGT事件.
推断的基质特异性和硫化模式基于比较的基因组学.

结论:

藻类CHST表现出多样化的进化轨迹,包括血统特定的多样化和HGT.
已识别的CHST及其域结构为我们提供了关于不同藻类系的多种SPs生物合成的见解.
这项研究弥合了藻类CHST功能和进化的知识差距,为未来的实验验证铺平了道路.

关键词:

藻类藻类是一种藻类.进化进化进化进化进化进化进化功能功能功能功能横向的基因转移是可以实现的.人类的家谱 (Phylogeny) 是一个学科.硫酸多糖化合物硫酸多糖化合物

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