作为抗生素持续释放载体的高级血小板丰富纤维素 (A-PRF)
Karim Fawzy El-Sayed1, Christian Flörke2, Gina Jaber3
1Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany; Oral Medicine and Periodontology Department, Faculty of Dentistry, Cairo University, Cairo, Egypt.
概括
先进的富血小板纤维素 (A-PRF) 在10天内有效地提供诸如西普罗夫洛克萨和青素/美特罗尼达之类的抗生素. 这项研究表明A-PRFF.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 传染病研究 传染病研究
背景情况:
- 富含血小板纤维素 (PRF) 是一种具有再生性质的血液缩物.
- 当地的抗生素输送旨在提高疗效并减少系统性副作用.
- 先进的富血小板纤维素 (A-PRF) 作为持续释放抗生素的载体的潜力需要进一步研究.
研究的目的:
- 评估A-PRF作为青素/甲尼达和西普罗夫洛克萨的持续释放载体.
- 评估抗生素加载的A-PRF对聚合菌 (Aggregatibacter actinomycetemcomitans (Aa)) 的抗菌活性.
- 从A-PRF中描述抗生素的释放动力学.
主要方法:
- 准备的A-PRF是用青素/美特罗尼达 (P+M) 或西普洛素 (C) 制备的.
- 进行了组织学和扫描电子显微镜 (SEM) 分析.
- 使用抗生素测试对 Aa 的抗微生物活性进行了测试.
- 使用ELISA确定了抗生素释放动力学.
主要成果:
- 装有抗生素的A-PRF保持了它的结构完整性.
- 在7天的时间里,A-PRF/C和A-PRF/P+M对Aa表现出显著的抗菌活性.
- 埃利萨检测显示,A-PRF从A-PRF中持续释放10天的西普洛素和青素/美特罗尼达,青素显示稳步增加.
结论:
- A-PRF作为一个有前途的局部输送系统,持续释放抗生素.
- 这项技术有可能用于需要扩展抗微生物作用的临床应用.
- 在管理局部感染方面,A-PRF具有显著的潜力.
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