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使用生物相容的固相微提取与通过微流体开放接口的质谱学相结合,在人体血中对化物和多化物物质进行高通量选

Yaping Li¹, Emir Nazdrajić², Wei Zhou²

¹Department of Chemistry, University of Waterloo, Waterloo, ON, N2L 3G1, Canada; Department of Chemistry, Capital Normal University, 105 West Third Ring Road North, Haidian District, Beijing, 100048, China.

Analytica chimica acta

|January 9, 2026

概括

一种使用固态微提取-微流体开放接口质谱法 (SPME-MOI-MS) 的新方法快速选人体血中的18个per-和多甲基物质 (PFAS). 这种高通量技术有助于大规模的生物监测和暴露评估.

科学领域:

环境化学环境化学
分析化学分析化学
毒理学毒理学毒理学

背景情况:

和多醇基物质 (PFAS) 是持久的,生物累积的环境污染物,与有害健康影响有关.
大规模的监测和暴露评估需要有效的,高通量PFAS的分析方法.
目前的分析方法可能耗时,限制了大规模生物监测工作.

研究的目的:

开发一个快速,高通量分析平台,用于查人体血中的PFAS.
为大规模PFAS暴露评估建立一个可持续的方法.
评估一种新型的SPME涂层,以有效地从生物基质中提取和清理PFAS.

主要方法:

开发一个固相微提取-微流体开放接口质谱 (SPME-MOI-MS) 平台.
使用一种新型的SPME涂层 (PAN粘合剂中的HLB-WAX颗粒) 来直接分析人体血.
绕过传统的液态色谱分离,以实现快速样品吞吐量.

主要成果:

每个样品的平均分析时间为3.7分钟,证明了高吞吐量能力.
新型的SPME涂层提供了复杂的生物基质的有效提取和清理.
对于18种目标PFAS化合物,已证明出色的线性 (1-100 ng/mL) 和低检测极限 (0.11-0.86 ng/mL).
在人体血样本中成功检测到PFOA和PFNA.

结论:

SPME-MOI-MS平台提供了一种快速有效的方法,用于在人体血中查PFAS.
这种方法适用于大规模的生物监测和长期暴露评估研究.
开发的方法显示了推动PFAS研究和公共卫生监测的巨大潜力.

关键词:

人类血是人类的血.质谱测量质量谱测量微流体开放接口的微流体接口固相微提取固相微提取方法通过和多基基物质.

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