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分析和识别与PRV FA和FB菌株之间的毒性差异相关的circRNA调节的ceRNA网络

Qiu-Yong Chen^1,2,3, Wen-Juan Zhao¹, Dong-Lin Wang¹

¹College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

BMC veterinary research

|January 9, 2026

概括

宿主循环RNAs (circRNAs) 影响伪狂犬病病毒 (PRV) 感染. 四个差异表达的circRNA与细胞亡和溶酶体通路有关,影响PRV病毒性和复制差异.

科学领域:

病毒学病毒学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

越来越多地认识到宿主循环RNA (circRNA) 和它们在病毒感染中的作用.
伪狂热病毒 (PRV) 感染中circRNAs的特定功能,特别是关于不同菌株的功能,仍未得到充分探索.
病毒性和PRV的复制受病毒基因和宿主非编码RNA的影响.

研究的目的:

为了研究感染病毒性 (FA) 和弱化 (FB) PRV菌株的宿主细胞中的circRNA表达特征.
构建一个竞争的内源RNA (ceRNA) 网络,涉及差异表达的circRNAs (DE circRNAs),microRNAs (miRNAs) 和信使RNAs (mRNAs).
预测DE circRNAs的生物功能及其在PRV病变发生中的潜在作用.

主要方法:

使用高通量测序来分析circRNA表达特征.
生物信息分析被用来建立一个ceRNA网络.
在DE circRNAs的目标基因上进行了功能丰富分析.

主要成果:

在受PRV感染的细胞中发现了四种DE circRNAs (animalcirc_014421,animalcirc_004115,animalcirc_010807和animalcirc_000091).
这些DE circRNAs在涉及miRNAs和mRNAs的ceRNA网络中的相互作用中介.
与这些DE circRNAs相关的目标基因在亡和溶酶体相关的途径中得到丰富,包括简单疹病毒1感染.

结论:

主体circRNAs,特别是已识别的DE circRNAs,可能有助于在PRV FA和FB菌株之间观察到的毒性和复制的差异.
亡和溶酶体调节途径被认为是circRNAs调节PRV表型差异的关键机制.
这项研究提供了新的分子洞察力,了解宿主circRNAs如何影响PRV的病原性.

关键词:

这就是CeRNA.循环RNAs 是一个循环RNA.PRVV 是一个很好的选择.复制复制复制复制复制复制复制病毒性病毒性

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