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在Macrobrachium rosenbergii中建立基于CRISPR/Cas9的基因组编辑系统

Junjun Yan¹, Siyu Qian^1,2, Guo Li¹

¹Xianghu Laboratory, Hangzhou 311231, China.

Animals : an open access journal from MDPI

|January 10, 2026

概括

研究人员开发了一种高效的CRISPR/Cas9基因组编辑系统,用于巨型淡水 (Macrobrachium rosenbergii). 这一突破使得有针对性的基因改造能够在这个经济上重要的水产养殖物种中进行基因改进.

科学领域:

水产养殖和水生基因组学
基因编辑技术的技术

背景情况:

巨型淡水 (Macrobrachium rosenbergii) 是全球一个有价值的水产养殖物种.
由于缺乏有效的基因组编辑工作流程,M. rosenbergii的遗传改进受到阻碍.

研究的目的:

优化CRISPR/Cas9微注射系统,用于M. rosenbergii胚胎中的向突变发生.
建立功能基因组学和M. rosenbergii.基因改进的基础.

主要方法:

对一细胞M. rosenbergii胚胎的常规采集使用了受控交配策略.
确定了最佳的微注射时间 (受精后0.5-2小时) 和针尖大小 (~1微米).
应用CRISPR/Cas9来编辑眼睛发育基因MrPAX6和与性别相关的基因MrIAG.

主要成果:

通过优化微注射技术,早期胚胎死亡率降至约10%.
编辑MrPAX6导致46.9%的突变率和50%的眼睛色素区域减少.
编辑MrIAG实现了84%的突变率,大约有35%的化生存率.

结论:

一个高效和可复制的CRISPR/Cas9基因组编辑系统已经成功建立了M. rosenbergii.
这种优化的系统为未来的功能基因组学研究和M. rosenbergii的遗传增强计划提供了必不可少的工具.

关键词:

这就是CRISPR/Cas9的作用.马克罗布拉基乌姆罗森伯基基繁殖繁殖繁殖繁殖基因组编辑基因组编辑

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