在骨肌肉发育过程中对的IGF2BP2的分子表征和功能洞察力
Cui Wang1, Yi Liu1, Jiuli Dai2
1Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Animals : an open access journal from MDPI
|January 10, 2026
概括
我们描述了的胰岛素样生长因子2 mRNA结合蛋白2 (IGF2BP2),在肌肉组织中发现高表达. 细胞中的过度表达表明IGF2BP2在的骨肌肉发育中起着关键作用.
科学领域:
- 基因组学和分子生物学
- 动物科学动物科学
- 发展生物学 发展生物学
背景情况:
- 胰岛素样生长因子2 mRNA结合蛋白2 (IGF2BP2) 对新陈代谢,细胞增殖和瘤产生至关重要.
- 虽然其在肌肉发育中的作用在其他物种中已知,但的IGF2BP2功能尚未得到研究.
研究的目的:
- 克隆和鉴定的全长cDNA和基因组DNAIGF2BP2.2.
- 为了研究的IGF2BP2在不同组织和发育阶段的表达模式.
- 探索IGF2BP2在骨肌肉发育中的功能作用.
主要方法:
- 的全长cDNA和基因组DNA测序IGF2BP2.2.
- 鉴定遗传变异,包括框架转移突变.
- 在各种组织和发育阶段对IGF2BP2mRNA表达的定量分析.
- 在骨肌卫星细胞 (SMSCs) 中,IGF2BP2的过度表达,随后进行基因表达分析和蛋白质-蛋白质相互作用网络分析.
主要成果:
- 的IGF2BP2cDNA为2957bp,编码了一种553氨基酸蛋白质,具有五个RNA结合域. 基因组序列跨越12,183个基因组,有12个外子和11个内子.
- 确定了60种遗传变异,其中包括一个框架转移突变 (g.2299delG).
- 在雌的肝脏,心脏和肌肉组织中观察到高IGF2BP2mRNA水平在胚胎,生长和产卵阶段 (p <0.05).
- 在SMSC中,IGF2BP2的过度表达显著改变了参与肌肉发育和信号通路的基因表达,包括IGF1,EGFR,FGF19,BMP6,BMP2,ACVR1C和WNT5A的上调,以及MYBPC3,NODAL,HOXD13,TNXB和ADD2的下调 (Padj<0.01).
结论:
- 的IGF2BP2具有保留的RNA结合域,并表现出显著的遗传变异.
- 关键组织中的高表达表明的发育,特别是肌肉发育中的作用.
- IGF2BP2可以通过协调信号通路内的关键基因的表达来调节的骨肌肉发育.
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