植物基因网络的功能性表征揭示了长中花期调节中的关键FT1-AP1相互作用
Yuru Tang1,2, Yating Xu1,2, Haoming Mao1,2
1Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel, Switzerland)
|January 10, 2026
概括
长的开花是由DlFT1调节的,DlFT1与DlAP1相互作用,扩大已知的开花路径. 这项研究揭示了一种新的相互作用,DlFT1-DlAP1,在热带水果的开花调节.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 遗传学和基因组学 遗传学和基因组学
背景情况:
- 龙干 (Dimocarpus longan Lour.) 是一种长的植物. 是一个有价值的热带水果作物.
- 长安的开花时间由两个对立的FLOWERING LOCUS T (FT) 同类物调节.
- 长性FT基因与开花调节器FD和APETALA1 (AP1) 之间的相互作用尚不清楚.
研究的目的:
- 从长远来看,将与开花相关的基因DlFT1,DlFT2,DlAP1和DlFD分离和描述.
- 为了研究这些基因在花分化过程中的表达模式.
- 阐明长FT,FD和AP1同类体在开花调节中的相互作用网络.
主要方法:
- 隔离了四个与长期开花相关的基因.
- 在自然和诱导的花分化过程中,叶子和芽的表达形状.
- 在Arabidopsis thaliana中异质过度表达,亚细胞局部化试验,酵母双杂交和双分子光补充 (BiFC).
主要成果:
- 在长日条件下,DlAP1显著促进了Arabidopsis的早期开花.
- DlFT1与DlAP1直接相互作用,形成了一个新的调节节点.
- DlFD与DlFT1的相互作用比DlFT2更强;DlFT1与DlAP1相互作用,但不是DlFT2.
结论:
- DlFT1通过保留的FD-依赖途径和与DlAP1的新型直接相互作用促进长中的开花.
- 这些发现扩展了经典的FT-FD-AP1开花模型.
- 该研究提供了对长和其他多年木质物种的开花调节机制的重要见解.
更多相关视频
12:36Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
Published on: January 14, 2016
21.1K
07:55Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Published on: November 20, 2017
14.7K
相关概念视频
02:19Morphogenesis
30.2K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
30.2K
01:25Cell Signaling in Plants
6.1K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.1K
02:45Biological Clocks and Seasonal Responses
41.5K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
41.5K