一种新的方法来确定葡萄酒中的生物氨基
Anna Salimova1, Alexandra Vasilieva1, Evgeniy Belyaev1
1Disinfectology Institute of F.F. Erisman FSCH of Rospotrebnadzor, 18 Nauchny drive, 117246 Moscow, Russia.
Molecules (Basel, Switzerland)
|January 10, 2026
概括
一种新方法使用TsCl衍生物化和RP-HPLC精确确定葡萄酒中的生物氨基酸 (BA). 这种分析对于食品安全至关重要,因为BA可能会对健康产生不利影响.
科学领域:
- 分析化学 分析化学
- 食品化学 食品化学
- 生物化学 生物化学
背景情况:
- 生物胺 (BAs) 是一种在葡萄酒,奶酪和巧克力等食物中发现的化合物.
- 高摄入BA可以导致不良健康影响,如头痛和腹.
- 世界各地的监管机构监测和控制食品中的BA水平.
研究的目的:
- 开发和验证一种新的分析方法,用于同时确定葡萄酒中关键的生物原胺.
- 在复杂的食品矩阵中提高生物氨基胺分析的准确性和效率.
- 为葡萄酒行业提供可靠的质量控制和监管合规方法.
主要方法:
- 生物性胺的衍生 (普特瑞辛,尸体胺,组胺,提拉胺) 与p-toluene sulfonyl 化物 (TsCl) 的衍生.
- 使用硫酸盐 (K2S2O8) 来减轻分析期间的矩阵效应.
- 使用C8柱和二极管阵列探测器的逆相高性能液态染色学 (RP-HPLC) 进行分离和定量.
主要成果:
- 该方法在校准曲线 (r > 0.999) 中实现了极好的线性.
- 检测极限范围从0.00866毫克·L-1 (泰拉胺) 到0.346毫克·L-1 (希斯胺).
- 生物氨基的回收率在83.0%至110%之间,这表明其准确性很高.
结论:
- 拟议的RP-HPLC方法提供了高灵敏度,快速分析和简单的样本制备用于葡萄酒中的生物胺.
- 这种经过验证的方法适用于在红葡萄酒和白葡萄酒中同时量化布素,尸体素,基因组胺和提拉胺.
- 该方法支持对葡萄酒的有效质量控制和安全评估,以确定其生物氨基含量.
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