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磁性可回收的ICT功能化Fe₃O₄纳米粒子用于高效的马过氧化酶固定

Katarina Isaković¹, Marko Jonović², Dušan Sredojević¹

¹Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, Centre of Excellence for Photoconversion, University of Belgrade, 11001 Belgrade, Serbia.

Molecules (Basel, Switzerland)

|January 10, 2026

概括

磁铁纳米颗粒与5-氨酸功能化,使得有效的共价酶固定,创造强大的生物催化剂. 这种接口电荷转移策略为可重复使用的酶应用提供了卓越的性能.

科学领域:

材料科学材料科学材料科学
纳米技术纳米技术
生物化学生物化学

背景情况:

联体和金属氧化物之间的界面电荷转移 (ICT) 复合体促进了用于催化和生物结合的表面功能化.
磁铁 (Fe3O4) 纳米粒子提供磁性回收,使它们成为生物催化剂的有吸引力的支物.

研究的目的:

通过ICT复杂形成,使用联体5-aminosalicylic acid (5ASA) 和咖啡酸 (CA) 功能化磁石纳米颗粒.
在这些功能化纳米粒子上固定氧化酶 (HRP),用于催化应用.
评估固定酶系统的效率,活性和可重复使用性.

主要方法:

用5ASA和CA对Fe3O4纳米粒子进行表面修饰.
使用XRD,TEM和FTIR进行结构性表征.
通过pyrogallol氧化试验,对HRP的酶固定和催化活性的评估.
评估酶负载,固定效率和可重复使用性.

主要成果:

与Fe3O4/CA.相比,Fe3O4/5ASA-HRP表现出明显更高的催化活性 (20毫克支持每2.5个单位) 和固定效率 (高达1068毫克g-1),与Fe3O4/CA.相比.
Fe3O4/5ASA系统显示出优异的可重复使用性,并在多个循环中保持高活性.
生物催化剂的磁回收是快速而高效的.

结论:

在Fe3O4纳米颗粒上使用5ASA的基于ICT的功能化对于共价酶固定是非常有效的.
Fe3O4/5ASA平台为环境和工业应用提供了强大的和可重复使用的生物催化剂.
这种方法突显了定制纳米材料在先进生物催化剂中的潜力.

关键词:

5 - 氨基沙酸 (5ASA) 是一种5 - 氨基沙酸.在Fe3O4纳米粒子中.酶固定化酶固定化胡须过氧化酶 (HRP) 是一种接口电荷传输 (ICT) 接口

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