转录组动力学和产后肌肉发育的监管网络在莱州黑山羊中
Jiancheng Han1, Jing Huang2, Mengning Xu3
1Zhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524013, China.
International journal of molecular sciences
|January 10, 2026
概括
这项研究揭示了山羊肌肉生长过程中的动态基因表达和拼接,确定了关键途径和新型转录. 这些发现为通过分子育种改善山羊肉生产提供了见解.
科学领域:
- 动物基因组学 动物基因组学
- 文字转录学 (Transcriptomics) 是一个学科.
- 分子生物学分子生物学
背景情况:
- 羊的产后肌肉发育是复杂的,并未得到充分理解.
- 转录调节在肌肉成熟中起着至关重要的作用.
研究的目的:
- 在出生后发育期间,对莱州黑山羊的Longissimus dorsi转录组进行剖析.
- 识别动态基因表达,替代拼接和共同表达网络.
- 了解驱动肌肉成熟的转录重编程,并为繁殖提供资源.
主要方法:
- 长背肌肌的RNA测序 (RNA-Seq) 在出生,六个月和两岁时.
- 对基因表达模式,替代拼接和共同表达网络的分析.
- 功能丰富,加权基因共同表达网络分析 (WGCNA) 和蛋白质与蛋白质相互作用 (PPI) 分析.
- 定量实时PCR (qRT-PCR) 用于验证.
主要成果:
- 确定了动态基因表达和广泛的替代拼接事件.
- 在六个月到两年之间观察到显著的转录转移,随着增殖基因的下调和肌肉收缩/能量代谢途径的激活.
- 发现了905个新的转录,其中许多与线粒体功能有关.
- 强调PI3K-Akt,PPAR和信号通路的重要性.
- 鉴定了参与细胞循环和肌肉功能的枢纽基因.
- 验证了关键基因的时间表达 (例如,CYP4B1,HACD1,ACTC1).
结论:
- 转录性重编程指导产后山羊肌肉的发育.
- 小说的成绩单和已识别的路径提供了机械的见解.
- 这些发现为通过分子育种提高山羊肉生产提供了宝贵的遗传资源.
相关概念视频
Formation of Muscle Fibers from Myoblasts
5.8K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.8K
Master Transcription Regulators
7.7K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K
Regulation of Expression at Multiple Steps
1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Translational Regulation
523
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
523


