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尿液细胞外膀的试点蛋白质组分析支持"有毒尿液假说"作为耐火性IC/BPS病原体的恶性循环

Man-Jung Hung^1,2, Evelyn Yang^1,2, Tsung-Ho Ying^1,2

¹Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung 402306, Taiwan.

International journal of molecular sciences

|January 10, 2026

概括

在接受治疗的间歇性囊炎/膀疼痛综合征 (IC/BPS) 患者仍然有症状. 它们的尿液细胞外囊泡 (UEV) 显示慢性炎症,支持"有毒尿液假说",并建议新的治疗点.

科学领域:

泌尿器科泌尿器科泌尿器科泌尿器科
细胞生物学细胞生物学
蛋白质组学是指蛋白质组学.

背景情况:

间歇性囊炎/膀疼痛综合征 (IC/BPS) 是一种慢性疾病,尽管目前的治疗方法,但症状仍然持久.
尿液细胞外囊泡 (uEVs) 含有与疾病相关的分子信息,但它们在受治疗的IC/BPS中的蛋白质特征是未知的.

研究的目的:

在接受治疗的IC/BPS患者中研究UEVs的蛋白质特征.
测试"有毒尿液假说",该假说假定尿功能障碍,EV介导毒素透和炎症的循环.

主要方法:

从六名接受治疗的女性IC/BPS患者和四名健康对照人群中分离出了UEV.
蛋白质组学通过质谱学识别了差异表达的蛋白质.
功能验证涉及NF-κB记者测定和膀上皮细胞中的西部斑点.

主要成果:

IC/BPS uEVs显示了31个上调的蛋白质 (例如,HPGD,KRT8,14-3-3家族) 和19个下调的蛋白质 (例如,MPO,ELANE),表明抑制的急性中性粒细胞炎症.
与对照组相比,患者衍生的UEV显著增加了NF-κB激活.
在暴露于IC/BPS uEVs的膀上皮细胞中观察到14-3-3ζ和化NF-κB p65的上调.

结论:

这些发现支持治疗IC/BPS患者的"有毒尿液假说".
持续的NF-κB介导的慢性上皮压力发生,尽管急性炎症减少.
针对炎症和再生的疗法可能为IC/BPS提供新的治疗途径.

关键词:

间歇性囊炎/膀疼痛综合征 (IC/BPS) 的情况蛋白质组学蛋白质组学耐火性IC/BPS电路的使用情况.毒性尿液假设毒性尿液假设尿液中的细胞外囊泡.

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