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一种可视和快速的PCR测试条方法用于验证Sika鹿肉 (Cervus nippon) 的真实性

Lijun Gao¹, Yuxin Xie¹, Yating Zhang¹

¹School of Medical Technology, Beihua University, Jilin 132013, China.

International journal of molecular sciences

|January 10, 2026

概括

一个新的DNA测试条可以准确地识别西卡鹿肉,防止经济改. 这种快速的视觉聚合酶连锁反应 (PCR) 试验对市场完整性和消费者保护具有高度敏感性.

科学领域:

食品科学与技术食品科学与技术
分子生物学分子生物学
分析化学是一种分析化学.

背景情况:

升的西卡鹿肉价格增加了经济改风险.
需要快速,可靠的肉类认证方法来保护消费者和市场的完整性.

研究的目的:

开发一种新的,特定的,可视化的方法来识别Sika鹿肉.
创建一个实用的工具,用于现场验证肉类的真实性.

主要方法:

专门设计的原料向细胞染色体C氧化酶亚单元I (COI) 基因.
为PCR阳性对照构建了一个DNA标准等离子体.
开发了一种基于聚合酶链反应 (PCR) 的核酸试验条.

主要成果:

该试验显示高特异性和灵敏性 (1.0 ng·μL−1 DNA检测).
在混合样本中达到1%的Sika鹿肉的检测极限,降至0.1%.
真实样本产生了测试和控制线;伪造/负样本只产生了控制线.

结论:

开发的测试条是一个特定的,敏感的,用户友好的工具.
能够快速地在现场验证Sika鹿肉的真实性.
有效地打击肉类市场的经济改.

关键词:

通过DNA指纹检测.食品安全食品安全肉制品肉制品肉制品核酸试验条核酸试验条锡卡鹿是鹿的一种.

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