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诱导多能干细胞衍生的树突细胞为免疫调节益生菌的功能查提供了可靠的体外平台

Yin-Ling Chiang¹, Men-Yee Chiew², Sheng-Jye Lim²

¹Industrial Development Graduate Program of College of Engineering Bioscience, National Yang Ming Chiao Tung University, Hsinchu 300, Taiwan.

International journal of molecular sciences

|January 10, 2026

概括

人类诱导的多能干细胞衍生的树突细胞为选益生菌提供了可靠的方法. 某些益生菌菌株促进调节性T细胞分化,帮助免疫平衡.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
干细胞生物学干细胞生物学

背景情况:

益生菌调节免疫平衡,但使用原发性免疫细胞的常规查方法缺乏可复制性.
人类诱导的多能干细胞 (iPSCs) 为研究产生一致的免疫细胞提供了潜在的替代方案.

研究的目的:

评估iPSC衍生的树突细胞和益生菌菌株之间的免疫调节相互作用.
评估益生菌刺激的树突细胞对影响T细胞分化的潜力.

主要方法:

与五种益生菌菌株共同培养IPSC衍生的树突细胞.
人类CD4+T细胞暴露于来自共同培养的条件介质.
与酶相关的免疫吸收试验 (ELISA) 用于测量细胞因子的产生.

主要成果:

来自iPSC的树突细胞在对不同益生菌菌株的反应中表现出不同的细胞因子分泌特征.
三种菌株 (Lactiplantibacillus plantarum LPA-56,Limosilactobacillus reuteri RU-23,Lactobacillus fermentum Fem-99) 促进了调控性T细胞的分化.
来自iPSC的树突细胞在功能性益生菌查中表现出可靠性.

结论:

来自iPSC的树突细胞是评估益生菌免疫调节效应的可行和可复制模型.
这种方法有助于选择具有强大的免疫调节特性的益生菌候选者,特别是促进调节性T细胞分化.

关键词:

在CD4+ T细胞中,有CD4+ T细胞.共同文化是一种共同文化.树突细胞的树突细胞.诱导的多能干细胞干细胞.益生菌是一种益生菌.

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