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Updated: Jan 13, 2026

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库尔库米诺酸激活TET酶并增加白血病细胞中的DNA基甲基化和活性脱甲基化

Sridhar A Malkaram¹, Suhila Sawesi², Botao Peng³

¹Department of Mathematics, Engineering & Computer Science, West Virginia State University, Institute, WV 25112, USA.

International journal of molecular sciences

|January 10, 2026

概括

黄素及其模拟物二甲基黄素 (DMC) 激活TET酶,增加5-基甲基细胞素 (5hmC) 水平,并促进白血病细胞中的活性DNA脱甲基化. 这表明它有可能作为白血病的表观遗传疗法.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

库尔库米诺类药物,包括黄素和二甲氧黄素 (DMC),表现出各种药理性质.
这些化合物调节涉及DNA甲基化和基因素修饰的表观遗传酶.
库尔库米诺类药物对TET酶的影响,这对白血病中5-基甲基细胞因子 (5hmC) 调节至关重要,仍然在很大程度上未知.

研究的目的:

研究黄素和DMC对白血病细胞中TET酶异型的活性和表达的影响.
分析这些化合物对全球5hmC水平和分布的影响.
在单个CpG位点层面进行5hmC变化的全基因组评估.

主要方法:

氧化双硫酸盐测序被用来区分5-甲基细胞因和5hmC.
测量了TET酶活性和mRNA表达.
全球5hmC水平被量化.
在单个CpG分辨率下分析了全基因组5hmC分布.

主要成果:

黄素和DMC都显著增加了所有三种TET酶异型的活性和mRNA表达.
在用黄素和DMC治疗的白血病细胞中,全球5hmC水平升高.
全基因组分析揭示了药物诱导的5hmC增加和各种基因组区域的活性脱甲基化,包括促进体,CpG岛屿,外因子和内因子.
在数百个基因中,黄素和DMC调节了促进子5hmC和脱甲基化.

结论:

库尔库米诺类药物通过激活TET酶,作为活性脱甲基化剂.
这些发现为探索黄素与DNA低甲基化剂结合用于白血病治疗提供了科学基础.
这项研究突出了库尔库米诺酸在癌症治疗的表观遗传调制中的潜力.

关键词:

通过DNA氧甲基化进行DNA氧甲基化.这种酶是TET酶.活动脱甲基化.库尔库明是一种黄素.这是一种二甲氧化库尔库明 (dimethoxycurcumin).这种白血病是白血病.氧化双硫酸盐测序 (OxBS) 是一种

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