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从Limosilactobacillus reuteri LR1中检查氨酸合成酶A (CysK) 的结构功能检查

Anastasia A Pometun^1,2, Evgenii K Les^1,3, Alla V Chernobrovkina³

¹Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Avenue, 119071 Moscow, Russia.

International journal of molecular sciences

|January 10, 2026

概括

来自Limosilactobacillus reuteri LR1 (LreCysK) 的氨酸合成酶A (CysK) 分析了其生物化学特性. 这种酶表现出高稳定性和与其他CysKs的动态相似性,表明了潜在的生物技术应用.

科学领域:

生物化学生物化学
酶学是一种酶学.
微生物学微生物学

背景情况:

氨酸合成酶A (CysK) 对于L-氨酸的生物合成至关重要,支持细胞的基本功能.
从Limosilactobacillus reuteri LR1中获得LreCysK的基因之前已被克隆并表达为活性,可溶性酶.

研究的目的:

综合分析LreCysK的运动参数,热稳定性和结构特征.
探索LreCysK.的潜在生物技术应用.

主要方法:

用高压液态染色学 (HPLC) 和ninhydrin试验来确定酶动力学.
热稳定性通过差异扫描热度计进行评估.
研究了结构特征,包括活动部位分析.

主要成果:

LreCysK的动力参数 (K_M^OAS,k_cat) 与大肠杆菌和阿拉比多普西斯塔利亚纳的CysK相似.
该酶表现出高热稳定性,其点在添加Pyridoxal-5酸盐 (PLP) 后增加到近90°C.
结构分析显示LreCysK是具有类似于其他CysKs的活性位点的二元体,但具有独特的乳杆菌特异性特征和Ile50.0.等残留物.

结论:

LreCysK具有显著的生物化学多功能性和稳定性,特别是当与PLP复合时.
该酶的特性表明生物技术和医疗应用的潜力很大.
对LreCysK的进一步研究可以促进对硫代谢和酶工程的理解.

关键词:

晶体结构晶体结构半氨酸合成酶A的作用.酶活性的酶活性.乳酸细菌菌 (lactobacilli) 是一种乳酸菌.模型结构研究研究模型结构研究.医院内感染医院内感染

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