长读测序揭示了293T和A549细胞转录组中的细胞和特定状态的替代分离
Xin Li1,2, Hanyun Que2,3, Zhaoyu Liu2
1Institute for Chinese Medicine Frontier Interdisciplinary Science and Technology, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
International journal of molecular sciences
|January 10, 2026
概括
替代拼接 (AS) 对于细胞身份至关重要. 这项研究使用了长读测序来揭示和肺癌细胞中细胞特异性AS变化,揭示了新型异构体和独特的细胞反应.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 文字转录学 (Transcriptomics) 是一个学科.
背景情况:
- 替代拼接 (AS) 产生了转录基因多样性,影响了细胞身份.
- 细胞类型特定的AS,特别是对受体信号的反应,在像293T和A549这样的常见细胞系中并未完全理解.
- 了解AS的动态是解释细胞功能和疾病状态的关键.
研究的目的:
- 在基底和刺激条件下,在293T和A549细胞中描述细胞类型特定的替代拼接动态.
- 研究G蛋白结合受体 (GPCR) 过度表达对转录组复杂性和拼接模式的影响.
- 评估将长读序列与生物信息工具集成为全面的AS分析的实用性.
主要方法:
- 集成的牛津纳米孔长读序列与BGI短读序列.
- 在基底和GPCR过度表达条件下 (293T中的ADORA3,A549中的P2RY12) 配置了293T和A549细胞的转录组.
- 使用FLAIR和SQANTI3.3进行全长异型分析.
主要成果:
- 发现了广泛的转录组复杂性,在293T细胞中发现了18.02%的新异型,在A549细胞中发现了19.52%.
- 在293T细胞中观察到一个稳定的转录组,富含与拼接相关的途径.
- 在A549细胞中确定了与瘤发生过程相关的更广泛的转录重塑.
结论:
- 293T细胞是研究拼接调节的合适模型,因为它们具有稳定的转录组.
- A549细胞为探索瘤相关的转录组动态提供了一个相关的模型.
- 将长读序列与FLAIR/SQANTI3集成是剖析细胞状态特定的AS调节的有价值的.
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