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相关概念视频

Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Designing Growth Media for Bioreactors01:30

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
Scale-Up Processes01:14

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...

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相关实验视频

Updated: Jun 21, 2026

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
08:39

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开发一个外套式透气摇瓶,具有过程监测,控制和生物反应器样性能.

Vikash Kumar1,2, Chad Sundberg1,2, Venkatesh Srinivasan1

  • 1Center for Advanced Sensor Technology, University of Maryland Baltimore County, Baltimore, Maryland, USA.

Biotechnology and bioengineering
|January 10, 2026
PubMed
概括
此摘要是机器生成的。

一个新的外套透气瓶系统克服了传统摇瓶的局限性,通过实现实时气体控制. 这项创新显著提高了培养性能,提高了生物质和蛋白质产量,用于生物工艺开发.

关键词:
类似于生物反应器的瓶式.透气的摇瓶可以呼吸.一个外套的透气小瓶.监测和控制 监测和控制摇瓶生物反应器生物反应器

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相关实验视频

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科学领域:

  • 生物技术是生物技术.
  • 生物工艺工程 生物工艺工程
  • 细胞培养技术 细胞培养技术

背景情况:

  • 传统的摇瓶缺乏关键气体监测和控制 (溶解的氧气,二氧化碳).
  • 这种限制阻碍了早期生物工艺开发的优化和可扩展性.
  • 氧气限制和低于最佳的代谢状态是摇瓶培养中常见的问题.

研究的目的:

  • 引入和评估一个覆盖式透气瓶系统,以加强生物过程控制.
  • 评估实时气体控制对培养性能和代谢概况的影响.
  • 以高吞吐量,具有成本效益的格式展示类似生物反应器的能力.

主要方法:

  • 开发和实施一个覆盖式透气瓶系统.
  • 在各种介质和填充体积上培养微生物.
  • 实时监测溶解氧气,二氧化碳,pH和细胞外代谢物.
  • 培养性能 (生物质,蛋白质产量) 与传统瓶相比.

主要成果:

  • 该系统成功推迟了氧气限制,并改善了培养性能.
  • 与传统瓶相比,实现了超过150%的更高生物质和140%的更高的重组蛋白质产量.
  • 氧气敏感副产品的积累减少,表明呼吸代谢得到改善.
  • 证明了与水箱生物反应堆相比较的生物质积累和特定增长率.

结论:

  • 覆盖式透气瓶系统为上游过程优化提供了一个可扩展和可访问的平台.
  • 它以高吞吐量,低成本的格式提供了类似生物反应器的性能,加速了实验室规模的生物制造.
  • 这项技术提高了培养性能和代谢效率,克服了传统摇瓶的主要局限性.