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烯混合物在不同的人类细胞系中产生可变的协同和对抗作用
Lauren N Hart1,2, Katherine L Lev1,2, Sierra Hefferan2,3
1Program in Chemical Biology, University of Michigan, Ann Arbor, Michigan, USA.
菌有害藻类繁殖 (cyanoHABs) 生产的毒素威胁着健康. 这项研究表明,微囊 (MCs) 和亚纳贝诺 (APs) 的混合物比单个毒素带来更大的风险,影响风险评估.
科学领域:
- 环境毒理学环境毒理学
- 菌的花研究研究.
- 公共卫生风险 公共卫生风险
背景情况:
- 菌有害藻类繁殖 (cyanoHABs) 由于有毒的二次代谢产物,对人类,动物和生态系统健康构成重大风险.
- 蓝藻细菌属Microcystis以产生肝毒性微囊 (MCs) 和其他生物活性蓝藻 (如anabaenopeptins (APs)) 而闻名,这些在花中普遍存在.
- 在自然环境中同时出现的氨酸的综合毒理和生态影响尚不清楚.
研究的目的:
- 评估单个和组合的微囊 (MCs) 和亚纳贝诺 (APs) 对人类肺,和肝细胞活性的毒性影响.
- 评估Microcystis培养提取物,含有各种酸套件,对人类细胞活性的影响.
- 了解代谢物相互作用在Microcystis开花的整体毒性中的作用.
主要方法:
- 人类肺 (A549), (HK2) 和肝 (Hep-3B) 细胞系暴露于纯的MC和AP原体,它们的组合,以及微囊培养提取物.
- 使用剂量反应测定评估细胞活力.
- 分析Microcystis菌株中烯酸含量和与观察到的毒性相关性的分析.
主要成果:
- 个别的MC和AP原体在细胞系中表现出不同的毒性,其中MC-LA是最有毒的,其次是MC-LR,AP-A,AP-B和MC-RR.
- 对MC-LA和AP-B的联合暴露导致了对所有测试的人类细胞系的剂量依赖的协同毒性作用.
- 微囊培养提取物表现出细胞活力的显著,取决于菌株的减少,这表明毒性超出了单独的MC和AP.
结论:
- 环境相关的酸的混合物可能比单个化合物具有更大的毒性威胁.
- 微囊花的毒性受到各种代谢物的复杂相互作用的影响,而不仅仅是MC和AP.
- 未来的风险评估和对酸影响水域的管理策略必须考虑多种毒素和其他生物活性代谢物的协同作用.
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