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Updated: Jan 13, 2026

Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay
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Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

Published on: December 25, 2021

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FRET-FLIM:揭示植物中的转录因子相互作用

María Florencia Perotti1, Carlos Sánchez-Gómez1, Carmen Martin-Pizarro2

  • 1Fruit Ripening and Quality Group, Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM, UMA-CSIC), Málaga, Spain.

Methods in molecular biology (Clifton, N.J.)
|January 10, 2026
PubMed
概括

这项研究详细介绍了Förster共振能量转移与光终身成像显微镜 (FRET-FLIM) 协议相结合,用于可视化细胞中的蛋白质-蛋白质相互作用. 该方法使用光标签来检测体内转录因子之间的相互作用.

关键词:
同焦点显微镜的共聚焦显微镜弗雷特 - 弗利姆光体配对成为一对.在植物里,植物里.在生物体内,在生物体内.蛋白质与蛋白质之间的相互作用转录因子 转录因子

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