通过两阶段培养和适应性实验室进化来提高Tetraselmis chuii中的生物质和超氧化物脱酶产量
Zhen Ni Huang1, Sangkaran Pannerchelvan1, Murni Halim1,2
1Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Bioprocess and biosystems engineering
|January 10, 2026
概括
这项研究优化了Tetraselmis chuii的培养,以提高超氧化物脱酶 (SOD) 的产生. 一个两阶段的策略,介质优化和硫酸铜应激显著提高了微藻中的SOD活性.
科学领域:
- 生物技术是生物技术.
- 海洋生物学 海洋生物学
- 生物化学 生物化学
背景情况:
- 像Tetraselmis chuii这样的微藻类是天然抗氧化剂的宝贵来源.
- Tetraselmis chuii被欧盟认定为一种新食品.
- 氧化应激可以增强抗氧化剂的产生,但可能会阻碍生物质的生长.
研究的目的:
- 为了研究和优化超氧化物脱酶 (SOD) 在Tetraselmis chuii.的生产.
- 制定一种培养策略,平衡生物质积累和抗氧化剂过度生产.
- 提高微藻的抗氧化剂产量,用于潜在的食品和营养药物应用.
主要方法:
- 一个两阶段的培养策略:生物质优化,然后是受控的氧化应激诱导.
- 响应表面方法 (RSM) 用于优化关键介质组件 (NH4Cl,NaH2PO4,CuSO4,MnCl2).
- 应用各种压力因素 (重金属,氧化剂,盐度) 和适应性实验室进化 (ALE) 应激耐受性.
主要成果:
- 中等优化使生物质从416.67 mg/L增加到564.44 mg/L,SOD活性从1479.23 U/g增加到2757.27 U/g.
- 硫酸铜 (CuSO4) 在5.0 mg/L是最有效的压力,增加SOD活动到5774.76U/g.
- 经过27周的适应性实验室进化 (ALE) 实验,SOD活动增加了61%的菌株.
结论:
- 介质优化,压力诱导和ALE的综合方法对增强微藻抗氧化剂生产是有效的.
- 可以种植Tetraselmis chuii以最大限度地提高SOD产量,支持其新食品地位.
- 这项研究为微藻可扩展的抗氧化剂生产提供了一个框架.
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