作为一种化学工具,DNA Aptamer可以调节MEX3C介导的mRNA不稳定
Tongqing Li1,2, Jingjing Qin1,2, Zihao Huang2,3
1College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310000, China.
ACS applied bio materials
|January 10, 2026
概括
研究人员开发了一种DNA吸收体,MRiApt-PT-茎,以向RNA结合蛋白MEX3C. 这种探测器有效调节基因表达,恢复瘤抗原呈现,并为向无法治疗的癌症基因提供了一种新的策略.
科学领域:
- 分子生物学分子生物学
- 癌症研究 癌症研究
- 药物发现 药物发现 药物发现
背景情况:
- 通过mRNA周转,RNA结合蛋白 (RBPs) 控制基因表达.
- MEX3C是一种瘤特异性的RBP,它破坏了转录的稳定.
- 目前没有针对MEX3C的选择性工具.
研究的目的:
- 开发一种高亲和度的DNA吸收体,针对MEX3C.
- 创建一个用于调节MEX3C活动的化学探针.
- 为了研究MEX3C在癌症预后中的作用.
主要方法:
- 代阻断器-SELEX选择和序列优化.
- 胺酶结合亲和力和特异性的表征.
- 在基于细胞的测定和TCGA数据分析中评估阿巴胺功效.
主要成果:
- 开发了一种高亲和度DNA吸收体,MRiApt,针对MEX3C的KH1域.
- 一种稳定衍生品,MRiApt-PT-stem,显示出增强的稳定性和细胞吸收.
- MRiApt-PT干对抗了MEX3C,恢复了HLA-A2的表达,并改善了T细胞的识别.
- 高MEX3C表达与肝肝细胞癌的不良预后相关.
结论:
- MRiApt-PT-stem作为一个有价值的化学探针来研究MEX3C.
- 这种胺酶可以调节MEX3C介导的转录后调节.
- 这些发现支持针对MEX3C等RBP进行癌症治疗和转录基因组调节.
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