相关实验视频

Updated: Jan 13, 2026

Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples

Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples

Published on: October 18, 2019

来自食物传播疫情的Clostridium perfringens分离体的特征和比较基因组分析

Jiwei Liang¹, Juping Shi², Lan Xu²

¹School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, China.

International journal of food microbiology

|January 10, 2026

概括

这项研究调查了由Clostridium perfringens引起的食物传播疫情,使用全基因组测序 (WGS) 精确追踪来源. 粮食系统发现了粮食来源与临床病例之间的直接联系,改善了食品安全监督.

科学领域:

食品微生物学食品微生物学
基因组学就是基因组学.
流行病学流行病学

背景情况:

杆菌 (Clostridium perfringens) 是食物传播疾病的常见原因.
调查食源性疫情对公共卫生至关重要.
分子类型识别有助于识别病原体来源.

研究的目的:

为了调查与集体餐饮有关的食物传播疫情.
为了确定Clostridium perfringens分离的遗传关系.
评估全基因组测序 (WGS) 在疫情调查中的有效性.

主要方法:

收集了25个来自各种来源 (食物,环境,处理者,患者) 的Clostridium perfringens分离物.
进行了多位置序列类型化 (MLST),核心基因组MLST (cgMLST) 和单核酸多态化 (SNP) 分析.
使用全基因组测序 (WGS) 来进行高分辨率的类型化.

主要成果:

MLST确定了18种序列类型 (ST),其中包括14种新型.
WGS揭示了分离物之间的遗传多样性;F型菌株是同质的,A型菌株是异质的.
高分辨率cgSNP分析显示,食品和临床分离物之间存在密切的基因组联系 (15-50个SNP),特别是子和一个临床病例 (15个SNP).

结论:

WGS为食品传播疫情调查和精确来源追踪提供了卓越的分辨率.
将基因组学整合到食品安全监测中可以改善疫情应对和公共卫生.
该研究确定了特定食品和临床病例之间的潜在流行病学联系.

关键词:

宽豆宽豆是一种豆类.对比基因组分析子是一种.通过食物传播的病原体.分子类型的定型.

更多相关视频

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

相关概念视频

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...