CircCramp1l针对miR-532-3p/HMGB1/Drp1轴进行调节,以调节过敏性鼻炎的发生
Yalin Zhang1, Jiangang Wang2, Yilan Song2
1Jilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji 133002, PR China; Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Yanbian University, Yanji, PR China.
Biochemical pharmacology
|January 10, 2026
概括
像circCramp1l这样的循环RNAs (circRNAs) 通过调节HMGB1和Drp1来驱动过敏性鼻炎 (AR),影响线粒体功能和炎症. 针对这个circCramp1l/miR-532-3p/HMGB1/Drp1轴,为AR提供了一个新的治疗策略.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 过敏性鼻炎 (AR) 的发病因子尚未完全理解.
- 循环RNAs (circRNAs) 在AR进展中的作用需要进一步调查.
研究的目的:
- 在AR中阐明circRNA介导的调节机制.
- 确定AR的潜在治疗点.
主要方法:
- 收集了人类鼻腔粘膜和已确定的室内灰尘虫 (HDM) 诱导的AR小鼠和人类鼻上皮细胞 (HNEpC) 模型.
- 进行了circRNA/miRNA测序,GEO数据分析,双露西法酶记者测定,用质谱学进行Co-IP和分子动力学模拟.
- 使用条件淘汰,基因沉默,miRNA模仿和药物抑制 (Mdivi-1) 进行干预.
主要成果:
- 在AR中,HDM显著上调了circCramp1l,作为miR-532-3p的ceRNA,释放HMGB1.
- HMGB1直接结合Drp1,促进其酸化,导致线粒体裂变,ROS积累,并激活P2X7R/TLR4/NLRP3轴.
- 这一途径诱导了Th2极化,乙素透和上皮损伤,这些都是通过针对circCramp1l,miR-532-3p,HMGB1或Drp1.1的干预措施来逆转的.
结论:
- circCramp1l/miR-532-3p/HMGB1/Drp1轴通过线粒体动力学和炎症信号传递,为AR病变产生贡献.
- 这一途径为AR诊断和治疗提供了一个新的治疗点.
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