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基于乙型肝炎核心抗原的三价值VLP疫苗,用于预防猪病毒性腹
Shouzhi Sheng1, Yixue Sun2, Jiayi Qin1
1State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, and College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Vaccine
|January 11, 2026
概括
使用乙型肝炎病毒核心抗原 (HBcAg) 的新型三价病毒样颗粒 (VLP) 疫苗显示,它有望控制猪病毒性腹. 基于HBcAg的候选疫苗在小鼠中引起了强烈的细胞和幽默免疫反应.
科学领域:
- 兽医免疫学 兽医免疫学
- 疫苗开发 疫苗开发
- 分子病毒学分子病毒学
背景情况:
- 猪病毒性腹在猪业中造成重大经济损失.
- 目前针对TGEV,PEDV和PoRV的疫苗有制备和有效性的限制.
- 乙型肝炎病毒核心抗原 (HBcAg) VLPs是呈现外源抗原的有效平台.
研究的目的:
- 开发和评估一种用于猪病毒性腹的新型三价值VLP (triVLP) 候选疫苗.
- 在小鼠模型中评估基于HBcAg的triVLP的免疫性.
- 为了比较triVLP疫苗与商业疫苗的疗效.
主要方法:
- 连锁的截断和全长的HBcAg单体.
- 集成的TGEV,PEDV和PoRV中和表位体到截断的HBcAg变体中.
- 给BALB/c小鼠服用25微克和50微克剂量的triVLPs.
- 通过IFN-γ和IL-4水平评估细胞免疫力.
- 测量IgG和病毒中和 (VN) 抗体标位.
主要成果:
- 50微克的triVLP剂量显著增强了细胞免疫力,在免疫接种后35天 (dpi) 增加了IFN-γ和IL-4水平.
- 在35dpi时,50μg triVLP组与商业疫苗组相比,对TGEV,PEDV和PoRV的IgG和VN抗体标位显著更高.
- 基于HBcAg的triVLP诱导了强大的细胞和幽默免疫反应.
结论:
- 基于HBcAg的三价值VLP是猪病毒性腹的有希望的疫苗候选人.
- 开发的triVLP疫苗引起了强大的细胞和幽默免疫力.
- 这种新型疫苗平台为改善对猪病毒性疾病的控制提供了潜力.
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