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3D数字光处理生物打印和在现场区分hiPSC用于工程心脏组织

Aihik Banerjee¹, Kevin Dalsania¹, Jonah Damian²

¹Department of Bioengineering, University of California, Riverside, Riverside, CA 92521, USA.

Acta biomaterialia

|January 11, 2026

概括

研究人员开发了FiBGel,这是一种用于3D生物打印人类干细胞的新型生物墨水. 这一创新使得可以创建功能性心脏组织模型,提高可行性和可收缩性,用于疾病研究和药物开发.

科学领域:

生物材料科学生物材料科学
干细胞生物学干细胞生物学
组织工程是组织工程.

背景情况:

目前的人类心脏组织模型缺乏生理相关性,阻碍了心血管疾病研究和药物开发.
现有的二维培养和动物模型无法复制人类心肌的复杂性,而三维方法则在细胞生存和成熟方面扎.

研究的目的:

开发一种新的生物墨水,用于直接3D生物打印未分化的人类诱导多能干细胞 (hiPSCs).
为了在功能性工程心脏组织的生物打印结构中实现现场心脏差异化.

主要方法:

开发了FiBGel,这是一个可光交叉链接的,生物离子液体功能化的凝甲基烯 (GelMA) 水凝.
利用3D数字光处理 (DLP) 生物打印技术在FiBGel.Gel中封装hiPSC.
研究生物打印结构的 in situ 心脏分化和长期功能.

主要成果:

在FiBGel中证明了hiPSCs的成功3D DLP生物打印,创造了功能性心脏组织.
与2D方法相比,实现了优异的心脏生物标志物表达,持续的同步收缩性和长期活力 (长达4个月).
FiBGel表现出可调节的机械/电化学特性,高细胞兼容性和可打印性.

结论:

通过FiBGel,可以使用未分化的hiPSC进行先进的功能性人类心脏组织模型的生物制造.
这个平台为药理学查,疾病建模和再生医学提供了重大进展.
这项研究为没有外部支持的hiPSC衍生的心脏结构提供了前所未有的生存时间.

关键词:

3D DLP生物打印 3D DLP生物打印生物离子液体功能化水凝心脏组织工程心脏组织工程这就是hiPSCs.基于干细胞的3D心脏组织模型.

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