Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

16.0K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
16.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Apatite-doped cyanobacterial biochar for treating smelting site pollution: A "win-win" strategy for cyanobacteria resource recovery and heavy metal mitigation.

Environmental geochemistry and health·2026
Same author

The role of long non-coding RNA HCP5 in ulcerative cutaneous tuberculosis therapy introduction.

Frontiers in cellular and infection microbiology·2026
Same author

<i>In vitro</i> interspecies interactions between methicillin-resistant <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>: effects on bacterial growth, antibiotic susceptibility, and transcriptomic reprogramming.

Frontiers in cellular and infection microbiology·2026
Same author

Time trend of sugar-sweetened beverages intake from 2002 to 2022 among adults in Beijing, China.

Preventive medicine reports·2026
Same author

Nox1/4 inhibitor Setanaxib treatment ameliorates cardiac function in mouse models of Duchenne Muscular Dystrophy.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

The Association of Maternal Pre-Pregnancy Overweight with Childhood BMI Trajectories and Blood Pressure.

Healthcare (Basel, Switzerland)·2026

相关实验视频

Updated: Jan 13, 2026

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.4K

快速的植物内定向进化与GRAPE.

Xiangtan Chen1, Rui Deng2, Youjun Zhang3

  • 1State Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China; School of Advanced Interdisciplinary Sciences, University of Chinese Academy of Sciences, Beijing, China.

Trends in plant science
|January 11, 2026
PubMed
概括
此摘要是机器生成的。

研究人员开发了GRAPE (geminivirus replicon-assisted in planta directed evolution),这是植物进化的一个新平台. 这种方法通过将复制与蛋白质功能联系起来来克服瓶,从而使植物的高通量选成为可能.

关键词:
GRAPE (植物指导进化中的geminivirus复制器辅助)在植物中,有针对性的进化.吉米尼病毒复制器

更多相关视频

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

14.8K
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

31.1K

相关实验视频

Last Updated: Jan 13, 2026

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.4K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

14.8K
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

31.1K

科学领域:

  • 植物生物学 植物生物学
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 植物定向进化面临的瓶限制了吞吐量.
  • 现有的方法在选择过程中难以维持本地植物的信号和防御通路.

研究的目的:

  • 引入双胞胎病毒复制器辅助的植物定向进化 (GRAPE) 作为一个新的平台.
  • 克服植物进化工程中的局限性.
  • 在工厂中实现高通量功能选.

主要方法:

  • 使用双子病毒复制品用于植物内复制.
  • 与蛋白质功能相关的滚动循环复制 (RCR).
  • 采用复制品放大器进行选择.

主要成果:

  • GRAPE成功地弥补了植物导向的进化瓶.
  • 在植物中实现了微生物类的吞吐量,用于植物选择.
  • 在这个过程中保留了本地植物的信号和防御机制.

结论:

  • GRAPE为植物进化生物学和生物技术提供了一个强大的新工具.
  • 该平台促进了植物蛋白的快速功能选和工程.
  • 这种方法增强了对植物系统的研究和操纵.