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合理的分子设计,以提高ZHD101的热稳定性,基于在滴度界面引入二硫化物键和B因子分析

Shaoyan Zheng^1,2,3, Yujie Huang², Haiyi Zhang⁴

¹State Key Laboratory of Bioactive Molecules and Druggability Assessment, Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative Drugs, College of Life Science and Technology, Jinan University, Guangzhou 510632 Guangdong Province, China.

Journal of agricultural and food chemistry

|January 12, 2026

概括

来自Clonostachys rosea的Zearalenone水解酶 (ZHD101) 是为了增强热稳定性而设计的. 一个新的突变策略显著提高了它的稳定性,为动物料安全的工业应用铺平了道路.

科学领域:

生物技术是生物技术.
酵素工程是什么? 酶工程是什么
蛋白质化学蛋白质化学

背景情况:

泽拉莱是动物料中有害的真菌毒素.
泽拉伦水解酶 (ZHD101) 降解了泽拉伦,但其热稳定性较差,限制了其工业用途.

研究的目的:

通过合理的分子设计来增强泽拉伦水解酶 (ZHD101) 的热稳定性.
为工业应用开发一种有效的策略,以提高蛋白质的热稳定性.

主要方法:

理性分子设计涉及引入二硫化物键进行二分化.
分析原子位置波动和动态信息,以识别高振动热点.
虚拟和突变发生和构造自由能量计算,以创造精确的突变.

主要成果:

一种ZHD101变种 (ZHD101^T229C/D170C) 显示了显著改善的热稳定性.
该变体的半失活温度 (T₅₀) 增加了7°C,其半衰期 (t_1/2) 在50°C时翻了一番.
化温度 (T_m) 增加了18.1°C,是Zearalenone水解酶报告的最高温度.

结论:

一种涉及二硫化物键和B因子分析的组合突变策略有效地提高了ZHD101的热稳定性.
工程ZHD101^T229C/D170C变种适用于工业应用.
开发的策略可以作为修改和优化其他蛋白质的热稳定性的宝贵参考.

关键词:

这是一个ZHD101的ZHD101.这就是Dimer Dimer的意思.二硫化物债券是指二硫化物债券.理性的设计理性的设计.热稳定性的热稳定性

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