相关实验视频
Updated: May 11, 2026

10:55
Digital Microfluidics for Automated Proteomic Processing
Published on: November 6, 2009
12.9K
温度反应敏捷的基于糖的数字微流体:芯片内样本准备用于用于痕细胞和单细胞蛋白质组的质谱分析
Ling Yan1,2, Jiacheng Fang2, Huimin Zhang3
1Eastern Institute for Advanced Study, Eastern Institute of Technology, Ningbo 315100, China.
Analytical chemistry
|January 12, 2026
概括
我们为单细胞蛋白质组学开发了一个具有成本效益的数字微流体平台. 这种可访问的工作流简化了质谱测量样本处理,使得从微量样本中进行可靠的蛋白质识别.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 微流体学 微流体学
- 细胞生物学 细胞生物学
背景情况:
- 基于质谱 (MS) 的单细胞和微量细胞蛋白质组学提供了宝贵的细胞洞察力.
- 目前的方法受到高成本和复杂仪器的限制.
- 需要可访问,可扩展的工作流程,以实现常规的多业务.
研究的目的:
- 为单细胞和微细胞蛋白质组学提供一个具有成本效益和可访问性的工作流程.
- 为了展示一个可扩展的解决方案,与标准的MS平台兼容.
- 克服现有的微流体和细胞分类技术的局限性.
主要方法:
- 开发一个对温度敏感的基于阿加罗斯的数字微流体 (TRA-DMF) 平台.
- 在一个平行四通道格式的单步样品处理 (溶解,还原/化,消化).
- 实时可视化单细胞捕获和高样本回收 (98.3%) 的非pipetting转移.
主要成果:
- 该TRA-DMF系统使得有效的滴滴传输与MS兼容.
- 整个工作流程 (TRA-DMF-SCP) 需要大约3小时.
- 从50个细胞中识别出4000多个蛋白质组,使用高可重复性的Orbitrap Fusion MS.
结论:
- TRA-DMF平台为痕迹和单细胞蛋白质组学提供了一个实用且可扩展的解决方案.
- 这种工作流克服了油相微流体的MS不兼容性问题.
- 集成到常规的多学科工作流程中的潜力,以实现更广泛的可访问性.
相关概念视频
Steady Flow of a Fluid Stream
Consider a control volume, such as a pipe with solid boundaries, through which fluid flows and changes direction due to the impulse exerted by the resulting force from the pipe walls. In steady flow, the mass of fluid entering the control volume at a given time, t, with velocity v1, is equal to the mass leaving after infinitesimal time dt, with velocity v2.
During this process, the momentum of the fluid within the control volume remains constant over the time interval dt. By applying the...
During this process, the momentum of the fluid within the control volume remains constant over the time interval dt. By applying the...
Conservation of Mass in Moving, Nondeforming Control Volume
Stormwater detention basins are essential in managing runoff during heavy rainfall, particularly in urban areas where impervious surfaces increase the risk of flooding. Understanding the conservation of mass in these systems allows engineers to optimize basin performance, balancing inflow, outflow, and water storage.
In the context of a detention basin, the conservation of mass states that the total mass of water entering the basin must equal the mass leaving the basin plus any accumulation of...
In the context of a detention basin, the conservation of mass states that the total mass of water entering the basin must equal the mass leaving the basin plus any accumulation of...
Typical Model Studies
Fluid mechanics model studies often utilize scaled-down systems to predict fluid behavior in full-scale environments, such as river flows, dam spillways, and structures interacting with open surfaces. Maintaining Froude number similarity in river models is crucial, as it replicates surface flow features like wave patterns and velocities.
Design Example: Creating a Hydraulic Model of a Dam Spillway
Scaled hydraulic models of dam spillways provide a practical way to replicate and study the intricate flow dynamics of these structures. Often built to a 1:15 ratio, these models allow for observing critical water behavior, such as velocity distribution, flow patterns, and energy dissipation.
Gradually Varying Flow
Gradually varying flow (GVF) in open channels describes situations where water depth changes slowly along the channel due to factors like non-uniform bed slope, channel shape variations, or obstructions. This flow type occurs when the depth adjusts gradually to balance gravitational forces, shear forces, and energy requirements, resulting in a low rate of depth change.Characteristics of Gradually Varying FlowGVF is commonly observed in natural streams, rivers, and canals, where flow depth...
Rapidly Varying Flow
Rapidly varying flow (RVF) in open channels is characterized by abrupt changes in flow depth over a short distance, with the rate of depth change relative to distance often approaching unity. These flows are inherently complex due to their transient and multi-dimensional nature, making exact analysis difficult. However, approximate solutions using simplified models provide valuable insights into their behavior.Key Features of Rapidly Varying FlowRVF is commonly observed in scenarios involving...

