激活血小板和血小板衍生的微粒度:对象化血小板度和聚合血小板丰富的血血小板度之间的比较研究
Samaneh Sadeghi Neysiyani1, Sedigheh Amini-Kafiabad1, Ehteramolsadat Hossieni1
1Blood Transfusion Research Centre, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
概括
聚合的富于血小板的血缩剂 (PRP-PCs) 显示出与异位PCs相比的质量,在储存期间血小板激活和微粒生成较低. 这表明PRP-PCs是治疗性血小板剂量的可行替代品.
科学领域:
- 输血医学 输血医学
- 血液学 血液学 血液学
- 生物技术是生物技术.
背景情况:
- 治疗性血小板剂量通常需要多个单位的全血衍生或非血小板缩物 (PC).
- 评估血小板缩剂的替代制备方法对于优化输血疗效和安全至关重要.
研究的目的:
- 评估制备方法在储存期间对血小板微粒 (PMP) 计数和血小板激活的影响.
- 为了比较阿菲雷西斯-PCs与聚合的富血小板血PCs (PRP-PCs) 的质量.
主要方法:
- 准备和储存了四个阿菲雷西斯PC和四个聚合PRPPC.
- 血小板计数,pH,旋转,P-选择因表达 (血小板激活标志物) 和PMP计数在第1,3和第5天被评估.
- 还评估了白细胞 (WBC),红细胞 (RBC) 计数和细菌污染.
主要成果:
- 聚合的PRP-PCs在第5天显示了血小板数的显著下降.
- 在储存期间,PMP计数在聚合PRP-PC和非PC中显著增加.
- 阿菲雷西斯-PCs在第一天具有较低的白细胞/红细胞计数,但具有较高的P-选择素表达.
- 在第3天,阿菲雷西斯-PCs比聚合的PRP-PCs显示出更高的PMP计数.
- 聚合PRP-PC的质量标准与表化PC相美.
结论:
- 聚合的PRP-PC显示出可接受的临床使用质量,类似于非-PC.
- 聚合PRP-PCs在早期储存中表现出较低的血小板激活和PMP生成,而非化PCs则相比较低.
- 这表明,聚合PRP-PCs是治疗性血小板输血的有希望的替代方案.
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