从Spigelia anthelmia L. (Loganiaceae) 中发现,分离和杀菌活动的环氧化物
Toluwanimi E Akinleye1,2,3, Latifat O Sidiq2,4, Alfred Attah5
1Center for Physiology and Pharmacology, Medical University of Vienna, Schwarzspanierstr. 17, Vienna 1090, Austria.
Journal of natural products
|January 12, 2026
概括
研究人员发现了Spat1,一种来自Spigelia anthelmia的新型环氧化物,通过破坏膜来表现出强大的杀菌活性对* Bacillus subtilis. 这一发现扩大了已知的植物循环的来源,并揭示了专门的抗菌功能.
科学领域:
- 植物生物化学和分子生物学
- 抗微生物研究的研究.
- 自然产品的进化生物学.
背景情况:
- 环酸是来自植物的宏环酸,其特征是囊结的图案.
- 已知产生环酸的植物属于有限范围的形种类.
- 了解循环的多样性和演变对于发现天然产品至关重要.
研究的目的:
- 报道发现和描述一种新型环化物Spat1,来自*Spigelia anthelmia*.
- 扩大已知的环酸生产植物的遗传分布.
- 调查Spat1.1的抗菌活性和作用机制.
主要方法:
- 隔离,净化和Spat1.1的新序列.
- 对 * Bacillus subtilis * , * Staphylococcus aureus * 和 * Escherichia coli * 的杀菌活性的确定.
- 研究Spat1的作用机制,包括膜相互作用和结合研究.
- 使用AlphaFold AfCycDesign进行结构建模.
主要成果:
- 标记Spat1,一个30个残留的手类型的环氧化物,是从*Spigelia anthelmia* (Loganiaceae) 中鉴定出来的.
- 通过膜破坏,Spat1对*B. subtilis* (LC99.9=20μM) 显示出强大的杀菌活性.
- 斯帕特1对*黄金色*或*大肠杆菌* (LC99.9> > 400μM) 没有显著的活性.
- 针对*B. subtilis*的活性似乎独立于脂铁醇酸 (LTA),并且可能涉及细胞质膜脂.
- 结构建模揭示了一个典型的循环体β-sheet和310-helix架构.
结论:
- 在Spigelia anthelmia中发现Spat1扩大了生产环类植物的基因组范围.
- Spat1表现出专门的杀菌活性,主要是通过膜破坏对*B. subtilis*等格兰阳性细菌进行杀菌.
- 对*S. aureus*的选择性无活性归因于包括低净正电荷和膜组成在内的因素.
- Spat1有助于理解植物系的循环功能专业化和融合进化.
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